MAP1LC3A Recombinant Rabbit Monoclonal Antibody [SN07-40]
Catalog# ET1611-37
MAP1LC3A Recombinant Rabbit Monoclonal Antibody [SN07-40]
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WB
-
IHC-P
-
IP
-
Human
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Mouse
-
Rat
概述
产品名称
MAP1LC3A Recombinant Rabbit Monoclonal Antibody [SN07-40]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein of Human MAP1LC3A aa 1-103 / 121.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IP
分子量
Predicted band size: 14 kDa
阳性对照
HeLa treated with 50μM Chloroquine for 18 hours cell lysate, C2C12 cell lysate, C2C12 treated with 50μM Chloroquine for 18 hours cell lysate, C6 cell lysate, C6 treated with 50μM Chloroquine for 18 hours cell lysate, mouse brain tissue.
偶联
unconjugated
克隆号
SN07-40
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
-
1:500-1:2,000
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IHC-P
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1:50-1:200
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IP
-
Use at an assay dependent concentration.
靶点
功能
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3β) and MAP-light chain 3 alpha (MAP-LC3α), both of which are mammalian homologs of yeast Apg8, are subunits that can associate with either MAP-1A or MAP-1B. While MAP-LC3β is essential for autophagy and is associated with autophagosome membranes after processing, MAP LC3α is involved in the formation of autophagosomal vacuoles and is localized to the intracytoplasmic membrane. MAP LC3α is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver and skeletal muscle, but are absent in thymus and peripheral blood leukocytes.
背景文献
1. Koukourakis MI et al. Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct Subcellular Distribution Kinetics and Expression in Cancer Cell Lines. PLoS One 10:e0137675 (2015).
2. Kim JY et al. Expression of autophagy-related proteins according to androgen receptor and HER-2 status in estrogen receptor-negative breast cancer. PLoS One 9:e105666 (2014).
序列相似性
Belongs to the ATG8 family.
组织特异性
Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
翻译后修饰
The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.; The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.; Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.
亚细胞定位
Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule.
别名
ATG8E antibody
Autophagy-related protein LC3 A antibody
Autophagy-related ubiquitin-like modifier LC3 A antibody
LC3 antibody
LC3A antibody
MAP1 light chain 3 like protein 1 antibody
MAP1 light chain 3-like protein 1 antibody
MAP1A/1B light chain 3 A antibody
MAP1A/MAP1B LC3 A antibody
MAP1A/MAP1B light chain 3 A antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of MAP1LC3A on different lysates with Rabbit anti-MAP1LC3A antibody (ET1611-37) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 50μM Chloroquine for 18 hours cell lysate
Lane 3: C2C12 cell lysate
Lane 4: C2C12 treated with 50μM Chloroquine for 18 hours cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 50μM Chloroquine for 18 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 14 kDa
Observed band size: 14/16 kDa
Exposure time: 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MAP1LC3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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