Phospho-RSK1 (S380) Recombinant Rabbit Monoclonal Antibody [SC05-32]
Catalog# ET1610-28
Phospho-RSK1 (S380) Recombinant Rabbit Monoclonal Antibody [SC05-32]
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WB
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IF-Cell
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IF-Tissue
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IP
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
Phospho-RSK1 (S380) Recombinant Rabbit Monoclonal Antibody [SC05-32]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phospho-peptide corresponding to residues surrounding S380 of human RSK1.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IF-Tissue, IP
分子量
Predicted band size: 83 kDa
阳性对照
A431 cell lysates, Hela, A431, 293, Hela whole cell lysate, Hela (starved overnight) then treated with TPA (200nM 4h) cell lysate, NIH/3T3 whole cell lysate, NIH/3T3 (starved 24h) then Calyculin A (100nM 15min) cell lysate.
偶联
unconjugated
克隆号
SC05-32
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:5,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
靶点
功能
The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, are important signaling intermediates that mediate responses to a broad range of ligand-activated receptor tyrosine kinases. It has been established that Rsk-3 is not activated by MAP kinase in vitro, unlike Rsk-1 and Rsk-2. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. The Rsk family amino-terminal kinase domain is phosphorylated on Ser 227 by 3-phosphoinositide-dependent protein kinase-1 (PDK1), which increases the kinase activity of Rsk. In the carboxy-terminal kinase domain, Rsk-1 and Rsk-2 are autophosphorylated on Ser 380 and Ser 386, respectively, which mediates the docking of PDK1 to Rsk in order to promote phosphorylation of substrates, such as histone H3.
背景文献
1. Smadja-Lamère N et al. Insulin Activates RSK (p90 Ribosomal S6 Kinase) to Trigger a New Negative Feedback Loop That Regulates Insulin Signaling for Glucose Metabolism. J Biol Chem 288:31165-76 (2013).
2. Tangredi MM et al. The C-terminal kinase and ERK-binding domains of Drosophila S6KII (RSK) are required for phosphorylation of the protein and modulation of circadian behavior. J Biol Chem 287:16748-58 (2012).
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
翻译后修饰
Activated by phosphorylation at Ser-221 by PDPK1. Autophosphorylated on Ser-380, as part of the activation process. May be phosphorylated at Thr-359 and Ser-363 by MAPK1/ERK2 and MAPK3/ERK1.; N-terminal myristoylation results in an activated kinase in the absence of added growth factors.
亚细胞定位
Nucleus, Cytoplasm.
别名
90 kDa ribosomal protein S6 kinase 1 antibody
dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
dJ590P13.1 antibody
EC 2.7.11.1 antibody
HU 1 antibody
HU1 antibody
KS6A1_HUMAN antibody
MAP kinase activated protein kinase 1a antibody
MAP kinase-activated protein kinase 1a antibody
MAPK-activated protein kinase 1a antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of Phospho-RSK1 (S380) on different lysates with Rabbit anti-Phospho-RSK1 (S380) antibody (ET1610-28) at 1/500 dilution.
Lane 1: Hela whole cell lysate
Lane 2: Hela (starved overnight) then treated with TPA (200nM 4h) cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lane 4: NIH/3T3 (starved 24h) then Calyculin A (100nM 15min) cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-28) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
ICC staining of Phospho-RSK1 (S380) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of Phospho-RSK1 (S380) in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of Phospho-RSK1 (S380) in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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PPP1R14B-mediated phosphorylation enhances protein stability of RPS6KA1 to promote hepatocellular carcinoma tumorigenesis
期刊: Biochimica Et Biophysica Acta - Molecular Cell Research
DOI:
IF: 4.6
应用: WB
反应种属: Human
发表时间: 2024 Sep
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