TRAP80 Rabbit Polyclonal Antibody
Catalog# ER1918-02
TRAP80 Rabbit Polyclonal Antibody
-
WB
-
FC
-
Human
-
Mouse
-
unconjugated
概述
产品名称
TRAP80 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human TRAP80 181-280/651
种属反应性
Human, Mouse
验证应用
WB, FC
分子量
73 KDa
阳性对照
Ubiquitous.
偶联
unconjugated
产品特性
形态
Liquid
浓度
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-2000
-
FC
-
1μg/Test
靶点
功能
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
背景文献
暂无
亚细胞定位
Nucleus.
UNIPROT
别名
77-KD subunit antibody
Activator recruited cofactor 77 kDa component antibody
Activator-recruited cofactor 77 kDa component antibody
ARC77 antibody
Cofactor required for Sp1 transcriptional activation subunit 6 antibody
cofactor required for Sp1 transcriptional activation
subunit 6
77kDa antibody
CRSP complex subunit 6 antibody
CRSP6 antibody
展开图片
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Sample: MDA-MB-231 Cell (Human) Lysate at 40 ug
Primary: Anti-TRAP80 (ER1918-02) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kD
Observed band size: 73 kD -
Sample:
spleen(mouse) Lysate at 40 ug
Primary: Anti-TRAP80 (ER1918-02) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73kD
Observed band size: 75 kD -
Blank control (blue line): Hela(fixed with pre-warmed 4% paraformaldehyde for 30min at 37℃ and then permeabilized with 90% ice-cold methanol for 30 min on ice)
Primary Antibody (green line): Rabbit Anti-TRAP80 antibody (ER1918-02),ilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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