SKP2 Rabbit Polyclonal Antibody
Catalog# ER1916-62
SKP2 Rabbit Polyclonal Antibody
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WB
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ELISA
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IHC-P
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IF
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ICC
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Human
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Mouse
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Rat
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Cow
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Rabbit
概述
产品名称
SKP2 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human Skp2 351-424/424
种属反应性
Human, Mouse, Rat, Cow, Rabbit
验证应用
WB, ELISA, IHC-P, IF, ICC
分子量
48 KDa
阳性对照
293T Cell Lysate, Hela cell, human thyroid gland carcinoma tissue.
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-2000
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ELISA
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1:5000-10000
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IHC-P
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1:100-500
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IF
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1:100-500
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ICC
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1:100
靶点
功能
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.
背景文献
暂无
亚细胞定位
Cytoplasm. Nucleus.
UNIPROT #
别名
CDK2/Cyclin A associated protein p45 antibody
Cyclin A/CDK2 associated protein p45 antibody
Cyclin-A/CDK2-associated protein p45 antibody
F box protein Skp2 antibody
F box/LRR repeat protein 1 antibody
F-box protein Skp2 antibody
F-box/LRR-repeat protein 1 antibody
FBL 1 antibody
FBL1 antibody
FBXL 1 antibody
展开图片
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Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Placenta tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Human 293T cell lysates
Primary: Anti-SKP2 (ER1916-62) at 1/1,000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution
Predicted band size: 48 kDa
Observed band size: 46 kDa -
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37℃ for 20 min; Antibody incubation with (SKP2) polyclonal Antibody, Unconjugated (ER1916-62) 1:100, 90 minutes at 37℃; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37℃ for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SKP2) Polyclonal Antibody, Unconjugated (ER1916-62) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SKP2) Polyclonal Antibody, Unconjugated (ER1916-62) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点&同通路的产品
SKP2 Recombinant Rabbit Monoclonal Antibody [PSH06-22]
Application: WB
Reactivity: Human,Monkey
Conjugate: unconjugated
