CDKN1A/P21 Rabbit Polyclonal Antibody
Catalog# ER1906-07
CDKN1A/P21 Rabbit Polyclonal Antibody
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ELISA
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IHC-P
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FC
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IF-Tissue
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IF-Cell
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Human
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Mouse
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Rat
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Dog
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Rabbit
概述
产品名称
CDKN1A/P21 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human P21 21-100/164
种属反应性
Human, Mouse, Rat, Dog, Rabbit
验证应用
ELISA, IHC-P, FC, IF-Tissue, IF-Cell
分子量
18 KDa
阳性对照
Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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ELISA
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1:5000-10000
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IHC-P
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1:100-500
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FC
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1μg/Test
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IF-Tissue
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1:100-500
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IF-Cell
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1:100
发表文章中的应用
发表文章中的种属
靶点
功能
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
背景文献
暂无
亚细胞定位
Cytoplasmic and Nuclear.
UNIPROT #
别名
CAP20 antibody
CDK-interacting protein 1 antibody
CDKI antibody
CDKN1 antibody
Cdkn1a antibody
CDN1A_HUMAN antibody
CIP1 antibody
Cyclin Dependent Kinase Inhibitor 1A antibody
Cyclin-dependent kinase inhibitor 1 antibody
Cyclin-dependent kinase inhibitor 1A (P21) antibody
展开图片
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Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (P21) Polyclonal Antibody, Unconjugated (ER1906-07) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-P21 Polyclonal Antibody, Unconjugated(ER1906-07) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining -
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-P21 Polyclonal Antibody, Unconjugated(ER1906-07) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining -
Tissue/cell: human hepatoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-P21 Polyclonal Antibody, Unconjugated(ER1906-07) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining -
Blank control(blue): Hep G2 Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-TNFR1/FITC Conjugated antibody (ER1906-07 /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions. -
Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37℃ for 20 min; Antibody incubation with (CDKN1A/P21) Polyclonal Antibody, Unconjugated (bs-10129R) 1:100, 90 minutes at 37℃; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37℃ for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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FFAR4 improves the senescence of tubular epithelial cells by AMPK/SirT3 signaling in acute kidney injury
Author: Yang, L., Wang, B., Guo, F., Huang, R., Liang, Y., Li, L., Tao, S., Yin, T., Fu, P., & Ma, L.
PMID: 36450712
应用: IF
反应种属: Mouse
发表时间: 2022 Nov
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Citation
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Exploring and Verifying the Mechanism and Targets of Shenqi Pill in the Treatment of Nonalcoholic Steatohepatitis via Network Pharmacology and Experiments
Author: He, B., Chen, Z., Nie, Y., Luo, M., Xu, S., Yan, J., & Chen, Z.
PMID: 35733920
应用: WB
反应种属: Rat
发表时间: 2022 Jun
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Citation
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Protective Effect of Follicle-Stimulating Hormone on DNA Damage of Chicken Follicular Granulosa Cells by Inhibiting CHK2/p53
Author: Zhou, S., Zhao, A., Wu, Y., Bao, T., Mi, Y., & Zhang, C.
PMID: 35455970
应用: WB
反应种属: Hen
发表时间: 2022 Apr
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Citation
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