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CD86 Rabbit Polyclonal Antibody

RMB: 1500

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Catalog# ER1906-01

CD86 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Tissue

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

CD86 Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

KLH conjugated synthetic peptide derived from the middle of rat CD86:140-175/313 <Extracellular>

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P, IF-Tissue, FC

分子量

38 KDa

阳性对照

Expressed by activated B-lymphocytes and monocytes.

偶联

unconjugated

产品特性

形态

Liquid

存放说明

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

存储缓冲液

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500-2000

  • IHC-P

  • 1:100-500

  • IF-tissue

  • 1:100-500

  • FC

  • 2μg/Test

靶点

功能

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

背景文献

暂无

亚细胞定位

Cell membrane; Single-pass type I membrane protein.

UNIPROT

SwissProt: P42081 Human

SwissProt: P42082 Mouse

SwissProt: O35531 Rat

别名

Activation B7-2 antigen 3 antibody

Activation B7-2 antigen antibody

B-lymphocyte activation antigen B7-2 2 antibody

B-lymphocyte activation antigen B7-2 antibody

B7 2 antibody

B7 antibody

B7-2 antibody

B7.2 antibody

B70 antibody

B72 antibody

展开

图片

  • Sample: <br />Lane 1: HepG2 (Human) Cell Lysate at 30 ug<br />Lane 2: U937 (Human) Cell Lysate at 30 ug<br />Lane 3: Spleen (Rat) Lysate at 40 ug<br />Lane 4: Spleen (Mouse) Lysate at 40 ug<br />Lane 5: HL-60 (Human) Cell Lysate at 30 ug<br />Primary: Anti-CD86 (<a href="/products/ER1906-01" style="font-weight: bold;text-decoration: underline;">ER1906-01</a>) at 1/1000 dilution<br />Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution<br />Predicted band size: 72-74 kD<br />Observed band size: 72 kD

    Sample:
    Lane 1: HepG2 (Human) Cell Lysate at 30 ug
    Lane 2: U937 (Human) Cell Lysate at 30 ug
    Lane 3: Spleen (Rat) Lysate at 40 ug
    Lane 4: Spleen (Mouse) Lysate at 40 ug
    Lane 5: HL-60 (Human) Cell Lysate at 30 ug
    Primary: Anti-CD86 (ER1906-01) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 72-74 kD
    Observed band size: 72 kD

  • Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; <br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; <br />Incubation: Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated(<a href="/products/ER1906-01" style="font-weight: bold;text-decoration: underline;">ER1906-01</a>) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated(ER1906-01) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Alternative Products

metafield image

CD86 Recombinant Rabbit Monoclonal Antibody [SJ20-00]

Application: WB,IF-Cell,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

同靶点 & 同通路的产品

metafield image

CD86 Recombinant Rabbit Monoclonal Antibody [SJ20-00]

Application: WB,IF-Cell,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

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