PAS2 Rabbit Polyclonal Antibody
Catalog# ER1902-29
PAS2 Rabbit Polyclonal Antibody
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IF-Tissue
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IHC-P
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Arabidopsis thaliana
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unconjugated
概述
产品名称
PAS2 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within thaliana PAS2 aa 172-221 / 221.
种属反应性
Arabidopsis thaliana
验证应用
IF-Tissue, IHC-P
分子量
Predicted band size: 25 kDa
阳性对照
Arabidopsis thaliana.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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IF-Tissue
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1:50-1:100
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IHC-P
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1:50-1:200
靶点
功能
Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May be an anti-phosphatase that prevents CDKA-1 dephosphorylation and activation. Involved in the hormonal control of cell division and differentiation. Required for proliferation control of meristematic and non-meristematic cells. Negative regulator of the cell cycle.
背景文献
1. Yanovsky MJ. et. al. Missense mutation in the PAS2 domain of phytochrome A impairs subnuclear localization and a subset of responses. Plant Cell. 2002 Jul;14(7):1591-603.
序列相似性
Belongs to the very long-chain fatty acids dehydratase HACD family.
组织特异性
High expression in young seedlings, roots, root tips, flowers and young siliques. Lower levels in leaves and stems.
亚细胞定位
Nucleus, endoplasmic reticulum membrane, cytoplasm.
别名
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2Curated
3-hydroxyacyl-CoA dehydratase PASTICCINO 2
AtPAS2
HACD
HCD
Protein PEPINO
Protein tyrosine phosphatase-like protein
PAS2
PEP
At5g10480
展开图片
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Immunofluorescence staining of paraffin- embedded Arabidopsis thaliana using anti-PAS2 rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1902-29 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1/500 for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded Arabidopsis thaliana tissue using anti-PAS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-29, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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