CD1a Mouse Monoclonal Antibody [A3F9]
Catalog# EM1901-89
CD1a Mouse Monoclonal Antibody [A3F9]
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WB
-
IHC-P
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Human
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unconjugated
概述
产品名称
CD1a Mouse Monoclonal Antibody [A3F9]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within human CD1a aa 50-250.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 37 kDa
阳性对照
K562 cell lysates, human skin tissue.
偶联
unconjugated
克隆号
A3F9
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein G affinity purified.
应用稀释度
-
WB
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1:500-1:1,000
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IHC-P
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1:1,000
靶点
功能
This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternative splicing results in multiple transcript variants.
背景文献
1. Maciocia PM. et. al. Anti-CD1a CAR T cells to selectively target T-ALL. Blood. 2019 May.
2. Ferrufino-Schmidt MC. et. al. Is CD1a useful for leishmaniasis diagnosis in the New World? J Cutan Pathol. 2019 Jan.
组织特异性
Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.
亚细胞定位
Cell membrane, endosome membrane, membrane raft.
UNIPROT
别名
CD 1a antibody
CD1 antibody
CD1a antibody
CD1A Antigen antibody
CD1A antigen, a polypeptide antibody
CD1a molecule antibody
CD1A_HUMAN antibody
cluster of differentiation 1 A antibody
cortical thymocyte antigen CD1A antibody
differentiation antigen CD1 alpha 3 antibody
展开图片
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Western blot analysis of CD1a on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-CD1a antibody (EM1901-89) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-89) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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