DOT1L Recombinant Rabbit Monoclonal Antibody [PSH01-57]
Catalog# HA721700
DOT1L Recombinant Rabbit Monoclonal Antibody [PSH01-57]
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WB
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
DOT1L Recombinant Rabbit Monoclonal Antibody [PSH01-57]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human DOT1/KMT4 aa 1- 500.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 165 kDa
阳性对照
K-562 cell lysate, THP-1 cell lysate, HeLa cell lysate, 293T cell lysate, human testis tissue, mouse testis tissue, rat testis tissue.
偶联
unconjugated
克隆号
PSH01-57
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IHC-P
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1:200-1:1,000
靶点
功能
Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.
背景文献
1. Feng Q., Wang H., Ng H.H., Erdjument-Bromage H., Tempst P., Struhl K., Zhang Y. Methylation of H3-lysine 79 is mediated by a new family of HMTases without a SET domain. Curr. Biol. 12:1052-1058 (2002)
2. Min J., Feng Q., Li Z., Zhang Y., Xu R.-M. Structure of the catalytic domain of human DOT1L, a non-SET domain nucleosomal histone methyltransferase. Cell 112:711-723 (2003)
亚细胞定位
Nucleus.
UNIPROT
别名
Disrupter of telomere silencing protein 1 antibody
DOT 1 antibody
DOT1 antibody
DOT1 like antibody
DOT1 like histone H3 methyltransferase (S. cerevisiae) antibody
DOT1 like histone H3 methyltransferase antibody
DOT1 like histone H3K79 methyltransferase antibody
DOT1 like protein antibody
DOT1, S. cerevisiae, homolog of antibody
DOT1-like protein antibody
展开图片
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Western blot analysis of DOT1L on different lysates with Rabbit anti-DOT1L antibody (HA721700) at 1/1,000 dilution.
Lane 1: K-562 cell lysate (40 µg/Lane)
Lane 2: THP-1 cell lysate (40 µg/Lane)
Lane 3: HeLa cell lysate (40 µg/Lane)
Lane 4: 293T cell lysate (40 µg/Lane)
Predicted band size: 165 kDa
Observed band size: 180 kDa
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DOT1L antibody (HA721700) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721700) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-DOT1L antibody (HA721700) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721700) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-DOT1L antibody (HA721700) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721700) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"