DNAJC5 Rabbit Polyclonal Antibody
Rmb: 1500
Catalog# ER63138
DNAJC5 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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FC(Intra)
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Human
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Mouse
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Rat
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Green monkey
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Pig
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unconjugated
概述
产品名称
DNAJC5 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within human DNAJC5 aa 151-198.
种属反应性
Human, Mouse, Rat, Green monkey, Pig
验证应用
WB, IF-Cell, FC(Intra)
分子量
Predicted band size: 22 kDa
阳性对照
HeLa (Human cervical adenocarcinoma cell) cell lysate, THP-1 (Human acute monoblastic leukemia cell) cell lysate, Hep G2 (Human liver cancer cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, C6 (Rat glioma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Mouse brain (P7) tissue lysate, Rat brain tissue lysate, Pig brain tissue lysate.
偶联
unconjugated
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:2,000
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IF-Cell
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1:100
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FC(Intra)
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1:200
靶点
功能
DnaJ homolog subfamily C member 5, also known as cysteine string protein or CSP is a protein, that in humans encoded by the DNAJC5 gene. It was first described in 1990. In humans, the gene is located on the long arm of chromosome 20 (20q13.33) on the Watson (positive strand). The gene is 40,867 bases in length and the encoded protein has 198 amino acids with a predicted molecular weight of 22.149 kilodaltons (kDa). The weight of the mature protein is 34 kDa. This gene is highly conserved and found both in invertebrates and vertebrates. In humans, a pseudogene of this gene is located on the short arm of chromosome 8.
背景文献
1. Lee J et al. Safeguarding Lysosomal Homeostasis by DNAJC5/CSPalpha-Mediated Unconventional Protein Secretion and Endosomal Microautophagy. Front Cell Dev Biol. 2022 May
2. Chen C et al. DNAJC5 facilitates the proliferation and migration of lung adenocarcinoma cells by augmenting EGFR trafficking. Commun Biol. 2025 May
亚细胞定位
Cytoplasm, cytosol, Membrane, Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane , Melanosome, Cell membrane.
别名
CLN 4 antibody
CLN4 antibody
CLN4B antibody
CSP antibody
cysteine string protein alpha antibody
Cysteine string protein antibody
DJC5 antibody
DnaJ (Hsp40) homolog subfamily C member 5 antibody
DnaJ homolog subfamily C member 5 antibody
DNAJC 5 antibody
展开图片
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Western blot analysis of DNAJC5 on different lysates with Rabbit anti-DNAJC5 antibody (ER63138) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate
Lane 3: Hep G2 (Human liver cancer cell) cell lysate
Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 5: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate
Lane 6: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Lane 7: C6 (Rat glioma cell) cell lysate
Lane 8: COS-1 (African green monkey kidney fibroblast) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER63138, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 22 kDa
Observed band size: 30 kDa -
Application: Immunocytochemistry (IF-cell)
Species: Rat
Sample: PC-12(Rat pheochromocytoma cell (undifferentiated))
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: ER63138, 1/100, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
☑ Relative expression (RE)
Western blot analysis of DNAJC5 on different lysates with Rabbit anti-DNAJC5 antibody (ER63138) at 1/2,000 dilution.
Lane 1: Mouse brain (P7) tissue lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat kidney tissue lysate
Lane 5: Pig brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 14 seconds; ECL: K1801
Negative expression of DNAJC5 protein in kidney is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER63138, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 22 kDa
Observed band size: 30 kDa -
Application: Flow Cytometry (Intra)
Species: Rat
Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated))
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: ER63138(1/200, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
DNAJC5 Recombinant Rabbit Monoclonal Antibody [PSH24-38]
Application: WB,IF-Cell,IHC-Fr,IHC-P,FC(Intra),IP
Reactivity: Human,Mouse,Rat,Green monkey,Pig
Conjugate: unconjugated
同靶点 & 同通路的产品
DNAJC5 Recombinant Rabbit Monoclonal Antibody [PSH24-38] - BSA and Azide free
Application: WB,IF-Cell,IHC-Fr,IHC-P,FC(Intra),IP
Reactivity: Human,Mouse,Rat,Green monkey,Pig
Conjugate: unconjugated
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