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iFluor™ 647 Conjugated Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338]

RMB: 3280

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Catalog# HA720145F

iFluor™ 647 Conjugated Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338]

Application

  • IF-Cell

  • IF-Tissue

Reactivity

  • Human

  • Mouse

概述

产品名称

iFluor™ 647 Conjugated Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human Cytokeratin 8 aa 321-370 / 483.

种属反应性

Human, Mouse

验证应用

IF-Cell, IF-Tissue

分子量

Predicted band size: 54 kDa

阳性对照

SK-Br-3, human breast tissue.

偶联

iFluor™ 647

克隆号

SU0338

RRID

AB_3072083

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • IF-Cell

  • 1:100

  • IF-Tissue

  • 1:200

靶点

功能

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. They have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 8 expression is seen in epithelium and epithelium-derived tumors. Cytokeratin 8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.

背景文献

1. Ruiz A. et al. Effect of hydroxychloroquine and characterization of autophagy in a mouse model of endometriosis. Cell Death Dis 7:e2059 (2016).

2. Xiao, L. et al. Three-dimensional epithelial and mesenchymal cell co-cultures form early tooth epithelium invagination-like structures: expression patterns of relevant molecules. J. Cell. Biochem. 113: 1875-1885(2012).

亚细胞定位

Nucleoplasm, Nucleus matrix, Cytoplasm.

UNIPROT #

SwissProt: P05787 Human

SwissProt: P11679 Mouse

别名

CARD2 antibody

Cell proliferation inducing gene 46 protein antibody

Cell proliferation inducing protein 46 antibody

CK 8 antibody

CK-8 antibody

CK8 antibody

CYK8 antibody

Cytokeratin 8 antibody

Cytokeratin-8 antibody

K2C8 antibody

展开

图片

  • Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 8 with Rabbit anti-Cytokeratin 8 antibody (<a href="/products/HA720145F" style="font-weight: bold;text-decoration: underline;">HA720145F</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 8 antibody (<a href="/products/HA720145F" style="font-weight: bold;text-decoration: underline;">HA720145F</a>, red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta III Tubulin (<a href="/products/M0805-8" style="font-weight: bold;text-decoration: underline;">M0805-8</a>, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/800 dilution.

    Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 8 with Rabbit anti-Cytokeratin 8 antibody (HA720145F) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 8 antibody (HA720145F, red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

    Beta III Tubulin (M0805-8, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution.

  • Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 8 (<a href="/products/HA720145F" style="font-weight: bold;text-decoration: underline;">HA720145F</a>) and Vimentin (EM0401).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (<a href="/products/HA720145F" style="font-weight: bold;text-decoration: underline;">HA720145F</a>, red) at 1/200 dilution and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.<br /><br />iFluor&trade; 488 conjugate-Goat anti-Mouse IgG (<a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 8 (HA720145F) and Vimentin (EM0401).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (HA720145F, red) at 1/200 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

    iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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