Cytokeratin 17 Recombinant Rabbit Monoclonal Antibody [SR45-06]
概述
产品名称
Cytokeratin 17 Recombinant Rabbit Monoclonal Antibody [SR45-06]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Cytokeratin 17 aa 1-50 / 432.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IF-Tissue, IHC-P
分子量
Predicted band size: 48 kDa
阳性对照
A431 cell lysates, Mouse skin tissue lysate, Rat skin tissue lysate, SiHa, human prostate tissue, human skin tissue, human tonsil tissue, mouse prostate tissue, human cervical carcinoma tissue.
偶联
unconjugated
克隆号
SR45-06
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:2,000
-
IF-Cell
-
1:50
-
IF-Tissue
-
1:200
-
IHC-P
-
1:50-1:1,500
靶点
功能
Cytokeratin 17 is a member of the Cytokeratin subfamily of intermediate filament proteins (IFPs). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. Cytokeratin 17 contains 432 amino acids and is expressed in the nail bed, hair follicle, sebaceous glands and other epidermal appendages. Cytokeratin 17 functions to regulate cell growth and size through its interactions with the adaptor protein 14-3-3-sigma to mediate protein synthesis. Mutations in the gene encoding for Cytokeratin 17 lead to depressed protein translation and smaller sized skin keratinocytes, corresponding to decreased Akt/mTOR signaling activity. Cytokeratin 17 may be a useful marker for cervical stem cell identification, squamous cell carcinoma of the larynx, respiratory syncytial virus and transitional cell carcinomas of the human urinary tract.
背景文献
1. Doucet YS et al. The touch dome defines an epidermal niche specialized for mechanosensory signaling. Cell Rep 3:1759-65 (2013).
2. Johnson EK et al. Identification of new dystroglycan complexes in skeletal muscle. PLoS One 8:e73224 (2013).
序列相似性
Belongs to the intermediate filament family.
组织特异性
Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).
翻译后修饰
Phosphorylation at Ser-44 occurs in a growth- and stress-dependent fashion in skin keratinocytes, it has no effect on filament organization.
亚细胞定位
Cytoplasm.
别名
39.1 antibody
CK 17 antibody
CK17 antibody
CK-17 antibody
Cytokeratin-17 antibody
K17 antibody
K1C17_HUMAN antibody
Keratin 17 antibody
keratin 17 epitope S1 antibody
keratin 17 epitope S2 antibody
展开图片
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Western blot analysis of Cytokeratin 17 on A431 cell lysates with Rabbit anti-Cytokeratin 17 antibody (ET1602-16) at 1/2,000 dilution.
Lysates/proteins at 15 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-16) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Cytokeratin 17 on different lysates with Rabbit anti-Cytokeratin 17 antibody (ET1602-16) at 1/1,000 dilution.
Lane 1: Mouse skin tissue lysate
Lane 2: Rat skin tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-16) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of SiHa cells labeling Cytokeratin 17 with Rabbit anti-Cytokeratin 17 antibody (ET1602-16) at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 17 antibody (ET1602-16) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-16, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 17 antibody (ET1602-16) at 1/1,500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-16) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 17 antibody (ET1602-16) at 1/1,500 dilution.
The section was not undergone antigen retrieval. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-16) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-16, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-16, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-Cytokeratin 17 antibody (ET1602-16) at 1/1,500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-16) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-Cytokeratin 17 antibody (ET1602-16) at 1/1,500 dilution.
The section was not undergone antigen retrieval. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-16) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 17 (ET1602-16).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 17 (ET1602-16, red) at 1/200 dilution at +4℃ overnight, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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A Single-Cell Transcriptomic Atlas of Epithelial Cell Heterogeneity During the Crown-to-Root Transition in the Mouse Molar
期刊: International Journal Of Molecular Sciences
DOI: 10.3390/ijms27031162
IF: 4.9
应用: IF-tissue
反应种属: Mouse
发表时间: 2026 Jan
-
Structure-Based Optimization of Moracin M as Potent and Selective PDE4 Inhibitors with Antipsoriasis Effects
期刊: Journal Of Medicinal Chemistry
DOI: 10.1021/acs.jmedchem.5c00266
IF: 6.8
应用: WB
反应种属: Human
发表时间: 2025 Mar
-
Discovery of pinoresinol dimethyl ether as a natural PDE4 inhibitor with anti-psoriatic effects
期刊: Bioorganic Chemistry
DOI: 10.1016/j.bioorg.2025.109414
IF: 4.7
应用: IHC
反应种属: Mouse
发表时间: 2025 Dec
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