Cathepsin B Rabbit Polyclonal Antibody
Catalog# ER1905-48
Cathepsin B Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Tissue
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FC
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Human
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Mouse
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Rat
概述
产品名称
Cathepsin B Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human Cathepsin B heavy chain 251-339/339
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue, FC
分子量
23/28/37 KDa
阳性对照
Human esophageal carcinoma, Mouse Spleen Lysate.
偶联
unconjugated
产品特性
形态
Liquid
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-2000
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IHC-P
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1:100-500
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IF-tissue
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1:100-500
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FC
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1µg/Test
靶点
功能
Cathepsin B is synthesized on the rough endoplasmic reticulum as a preproenzyme of 339 amino acid with a signal peptide of 17 amino acids. Procathepsin B of 43/46 kDa is then transported to the Golgi apparatus and cathepsin B is formed. Mature cathepsin B is composed of a heavy chain of 25-26 kDa and a light chain of 5kDa, which are linked by a dimer of disulfide. Cathepsin B may enhance the activity of other protease, including matrix metalloproteinase, urokinase (serine protease urokinase plasminogen activator), and cathepsin D, and thus it has an essential position for in the proteolysis of extracellular matrix components, intercellular communication disruption, and reduced protease inhibitor expression. It is also involved in autophagy and catabolism, which is advantageous in tumor malignancy and is possibly involved in specific immune resistance.
背景文献
暂无
亚细胞定位
Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
UNIPROT
别名
Amyloid precursor protein secretase antibody
APP secretase antibody
APPS antibody
CATB_HUMAN antibody
Cathepsin B heavy chain antibody
Cathepsin B1 antibody
CathepsinB antibody
CPSB antibody
CTSB antibody
cysteine protease antibody
展开图片
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Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Cathepsin B (ER1905-48) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46/28 kD
Observed band size: 30 kD -
Blank control: RSC96(blue). Primary Antibody:Rabbit Anti- Cathepsin B antibody(ER1905-48), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Antibody (ER1905-48, 5μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of ER1905-48 at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
Cathepsin B Mouse Monoclonal Antibody [J11-A11]
Application: WB,IF-Cell
Reactivity: Human,Zebrafish,Mouse,Rat
Conjugate: unconjugated
Cathepsin B Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human
Conjugate: unconjugated
