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CD68 Recombinant Antibody [PSH06-65] - Guinea pig IgG2 (Chimeric)

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Catalog# HA601595

CD68 Recombinant Antibody [PSH06-65] - Guinea pig IgG2 (Chimeric)

Application

  • WB

  • IHC-Fr

  • IHC-P

  • IF-Tissue

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

概述

产品名称

CD68 Recombinant Antibody [PSH06-65] - Guinea pig IgG2 (Chimeric)

抗体类型

Recombinant Chimeric Antibody

免疫原

Recombinant protein within mouse CD68 aa 1-316.

种属反应性

Mouse, Rat

验证应用

WB, IHC-Fr, IHC-P, IF-Tissue

靶点分子量

Predicted band size: 35 kDa

阳性对照

RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate.

偶联

unconjugated

克隆号

PSH06-65

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:2,000

  • IHC-Fr

  • 1:200

  • IHC-P

  • 1:2,000

  • IF-Tissue

  • 1:200

靶点

功能

CD68 (Cluster of Differentiation 68) is a protein highly expressed by cells in the monocyte lineage (e.g., monocytic phagocytes, osteoclasts), by circulating macrophages, and by tissue macrophages (e.g., Kupffer cells, microglia). Other names or aliases for this gene in humans and other animals include: CD68 Molecule, CD68 Antigen, GP110, Macrosialin, Scavenger Receptor Class D, Member 1, SCARD1, and LAMP4. The mouse equivalent is known as "macrosialin".

背景文献

1. Choudhary M et al. CD68: Potential Contributor to Inflammation and RPE Cell Dystrophy. Adv Exp Med Biol. 2023

2. Deng R et al. Periosteal CD68(+) F4/80(+) Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-beta1. Adv Sci (Weinh). 2022 Jan

亚细胞定位

Endosome membrane, Lysosome membrane; Cell membrane.

UNIPROT

SwissProt: P31996 Mouse

Entrez Gene: 287435 Rat

别名

CD 68 antibody

CD68 antibody

CD68 antigen antibody

CD68 molecule antibody

CD68_HUMAN antibody

DKFZp686M18236 antibody

gp11 antibody

Gp110 antibody

LAMP4 antibody

Macrophage antigen CD68 (microsialin) antibody

展开

图片

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of CD68 on different lysates with Guinea pig anti-CD68 antibody (<a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>) at 1/2,000 dilution.<br /><br />Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate (10 µg/Lane)<br />Lane 2: RAW264.7 treated with PNGase F cell lysate (10 µg/Lane)<br />Lane 3: Mouse spleen tissue lysate (20 µg/Lane)<br />Lane 4: Rat spleen tissue lysate (20 µg/Lane)<br /><br />Exposure time: 20 seconds; ECL: K1801<br /><br />CD68 is a glycoprotein and can be deglycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID : 7680921)<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Rabbit anti-Guinea pig IgG-HRP (<a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 35 kDa<br />Observed band size: 60-100 kDa

    ☑ Cell treatment (CT)

    Western blot analysis of CD68 on different lysates with Guinea pig anti-CD68 antibody (HA601595) at 1/2,000 dilution.

    Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate (10 µg/Lane)
    Lane 2: RAW264.7 treated with PNGase F cell lysate (10 µg/Lane)
    Lane 3: Mouse spleen tissue lysate (20 µg/Lane)
    Lane 4: Rat spleen tissue lysate (20 µg/Lane)

    Exposure time: 20 seconds; ECL: K1801

    CD68 is a glycoprotein and can be deglycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID : 7680921)

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA601595, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Rabbit anti-Guinea pig IgG-HRP (HA1021), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 35 kDa
    Observed band size: 60-100 kDa

  • Application: Immunofluorescence (IHC-Fr) <br /> <br />Species: Mouse <br />Tissue: Spleen <br />Sample: Frozen section <br /> <br />Antigen retrieval: Not required <br /> <br />Wash buffer: 1× PBST <br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/200, overnight at 4°C. <br />Secondary antibody: Goat Anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Spleen
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× PBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/200, overnight at 4°C.
    Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IHC-Fr) <br /> <br />Species: Rat <br />Tissue: Spleen <br />Sample: Frozen section <br /> <br />Antigen retrieval: Not required <br /> <br />Wash buffer: 1× PBST <br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/200, overnight at 4°C. <br />Secondary antibody: Goat Anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Rat
    Tissue: Spleen
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× PBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/200, overnight at 4°C.
    Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Mouse<br />Tissue: Liver<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/2,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1021, 30 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Mouse<br />Tissue: Spleen<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/2,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Spleen
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1021, 30 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Rat<br />Tissue: Liver<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/2,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1021, 30 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Rat<br />Tissue: Spleen<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/2,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Spleen
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1021, 30 minutes at room temperature.

  • Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Mouse <br />Tissue: Spleen <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× PBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/200, overnight at 4°C. <br />Secondary antibody: Goat Anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Spleen
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/200, overnight at 4°C.
    Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Rat <br />Tissue: Spleen <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× PBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/200, overnight at 4°C. <br />Secondary antibody: Goat Anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Rat
    Tissue: Spleen
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/200, overnight at 4°C.
    Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.

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