CD48 Recombinant Rabbit Monoclonal Antibody [JE37-98]
Catalog# HA721371
CD48 Recombinant Rabbit Monoclonal Antibody [JE37-98]
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WB
-
IHC-P
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Human
-
unconjugated
概述
产品名称
CD48 Recombinant Rabbit Monoclonal Antibody [JE37-98]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human CD48 aa 144-243 / 243.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 28 kDa
阳性对照
Raji cell lysate, Ramos cell lysate, Daudi cell lysate, Jurkat cell lysate, human colon tissue, human kidney tissue.
偶联
unconjugated
克隆号
JE37-98
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
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IHC-P
-
1:1,000
靶点
功能
CD48 is a glycosylphosphatidylinositol (GPI) -anchored membrane protein of the signaling lymphocyte activation molecule (SLAM) family, also known as SLAMF2 and BLAST-1. It is constitutively expressed on most hematopoietic cells (not on neutrophils and a subset of long-term hematopoietic stem cells in mice) and can be upregulated under certain conditions like infection. Interaction with its low affinity ligand CD2 promotes adhesion and TCR signaling. Interaction with the high affinity ligand CD244 (2B4) regulates natural killer (NK) and CD8 T cell activation and cytolytic function.
背景文献
1. Hawash I et al. The oxygen-substituted palmitic acid analogue, 13-oxypalmitic acid, inhibits Lck localization to lipid rafts and T cell signaling. Biochim. Biophys. Acta 1589:140-150 (2002)
2. Muhammad A et al. Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome. J. Immunol. 182:7672-7680 (2009)
亚细胞定位
Cell membrane, Membrane raft, Secreted.
别名
Antigen CD48 antibody
B cell membrane protein antibody
B lymphocyte activation marker BLAST 1 antibody
B-cell activation marker antibody
B-lymphocyte activation marker BLAST-1 antibody
BCM 1 surface antigen antibody
BCM1 antibody
BCM1 surface antigen antibody
BLAST 1 antibody
BLAST antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of CD48 on different lysates with Rabbit anti-CD48 antibody (HA721371) at 1/2,000 dilution.
Lane 1: Raji cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: THP-1 cell lysate (negative)
Lane 4: K-562 cell lysate (negative)
Lane 5: Ramos cell lysate
Lane 6: Daudi cell lysate
Lane 7: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 40-50 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721371) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CD48 antibody (HA721371) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD48 antibody (HA721371) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Reactivity: Human
Conjugate: unconjugated
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