BRG1 Recombinant Mouse Monoclonal Antibody [A5C9-R]
Catalog# HA601345
BRG1 Recombinant Mouse Monoclonal Antibody [A5C9-R]
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WB
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Human
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Mouse
-
Rat
概述
产品名称
BRG1 Recombinant Mouse Monoclonal Antibody [A5C9-R]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Synthetic peptide within human BRG1 aa 230-280.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 185 kDa
阳性对照
HeLa cell lysate, K-562 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, C6 cell lysate.
偶联
unconjugated
克隆号
A5C9-R
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
靶点
功能
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP.
背景文献
1. Park J.I. et. al. Telomerase modulates Wnt signalling by association with target gene chromatin. Nature 460:66-72(2009).
亚细胞定位
Nucleus.
别名
ATP dependent helicase SMARCA4 antibody
ATP-dependent helicase SMARCA4 antibody
BAF 190 antibody
BAF190 antibody
BAF190A antibody
Brahma protein like 1 antibody
BRG1 antibody
BRG1 associated factor 190A antibody
BRG1 protein antibody
BRG1-associated factor 190A antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of BRG1 on different lysates with Mouse anti-BRG1 antibody (HA601345) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: 293T cell lysate
Lane 4: A549 cell lysate (negative)
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 185 kDa
Observed band size: 200 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601345) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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