Alpha 1 Acid Glycoprotein Recombinant Rabbit Monoclonal Antibody [JE31-16]
Catalog# HA721396
Alpha 1 Acid Glycoprotein Recombinant Rabbit Monoclonal Antibody [JE31-16]
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WB
-
IHC-P
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Human
-
unconjugated
概述
产品名称
Alpha 1 Acid Glycoprotein Recombinant Rabbit Monoclonal Antibody [JE31-16]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Alpha 1 Acid Glycoprotein aa 51-100 / 201.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 23.5 kDa
阳性对照
Human small intestine tissue lysates, human liver tissue.
偶联
unconjugated
克隆号
JE31-16
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IHC-P
-
1:200
靶点
功能
Alpha-1-acid glycoprotein 1 is a protein that in humans is encoded by the ORM1 gene. This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression. ORM1 has been shown to interact with Plasminogen activator inhibitor-1.
背景文献
1. Joyce KE et al. Postexercise urinary alpha-1 acid glycoprotein is not dependent on hypoxia. J Appl Physiol (1985). 2022 Jan
2. Durmuş M et al. Does alpha-1-acid glycoprotein determine for infertility in polycystic ovary syndrome? Eur J Obstet Gynecol Reprod Biol. 2022 Jul
亚细胞定位
Secreted.
UNIPROT
别名
A1AG1_HUMAN antibody
AGP 1 antibody
AGP A antibody
AGP antibody
AGP1 antibody
Alpha 1 acid glycoprotein antibody
Alpha-1-acid glycoprotein 1 antibody
alpha-1-AGP antibody
Epididymis secretory sperm binding protein Li 153w antibody
glycoprotein, alpha-1-acid, of serum antibody
展开图片
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Western blot analysis of Alpha 1 Acid Glycoprotein on human small intestine tissue lysates with Rabbit anti-Alpha 1 Acid Glycoprotein antibody (HA721396) at 1/1,000 dilution.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 23.5 kDa
Observed band size: 45 kDa (Glycosylation)
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721396) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alpha 1 Acid Glycoprotein antibody (HA721396) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721396) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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