ATG12 Rabbit Polyclonal Antibody
Catalog# ER1802-82
ATG12 Rabbit Polyclonal Antibody
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WB
-
IHC-P
-
Human
概述
产品名称
ATG12 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within human Atg12 aa 1-120.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 15 kDa
阳性对照
A431 cell lysate, human kidney tissue lysate, 293T cell lysate, human liver cancer tissue, human colon tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
-
1:500-1:2,000
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IHC-P
-
1:50-1:200
靶点
功能
Atg12 (autophagy-related protein 12), also known as APG12, APG12L, FBR93 or HAPG12, is a 140 amino acid protein that is ubiquitously expressed and belongs to the Atg12 family of proteins. Atg12 is a homolog of the yeast protein Apg12 that participates in autophagy. Autophagy is a membrane trafficking mechanism that delivers cytoplasmic cargo to the vacuole/lysosome for degradation and recycling. In yeast, autophagy requires a protein conjugation system consisting of Apg12 covalently bound at the carboxy terminal glycine to lysine 149 of Apg5. Similarly in humans, Atg12 is essential for autophagy and localizes to the cytoplasm where it is covalently bound to APG5, a conjugation reaction that requires APG7, Atg10 and ATP. The Atg12-APG5 conjugate functions as an important regulator of the autophagic process and is required for the change in membrane morphology and development of autophagosomes. Due to alternative splicing events, two Atg12 isoforms exist.
背景文献
暂无
序列相似性
Belongs to the ATG12 family.
组织特异性
Ubiquitous.
翻译后修饰
Acetylated by EP300.
亚细胞定位
Cytoplasm, Membrane.
UNIPROT
别名
APG12-like antibody
APG12L antibody
ATG12 antibody
ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
ATG12 autophagy related 12 homolog antibody
ATG12_HUMAN antibody
Autophagy 12 antibody
Autophagy-related protein 12 antibody
FBR93 antibody
HAPG12 antibody
展开图片
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Western blot analysis of Atg12 on different lysates using anti-Atg12 antibody at 1/500 dilution.
Positive control:
Lane 1: A431 cell lysate
Lane 2: Human kidney tissue lysate
Lane 3: 293T cell lysate -
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Atg12 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Atg12 antibody. Counter stained with hematoxylin.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
ATG12 Recombinant Rabbit Monoclonal Antibody [JE30-61]
Application: WB,IF-Cell,FC
Reactivity: Human
Conjugate: unconjugated
同靶点 & 同通路的产品
ATG12 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
