ATF3 Recombinant Rabbit Monoclonal Antibody [PSH17-99]
Rmb: 666 特惠 1500
概述
产品名称
ATF3 Recombinant Rabbit Monoclonal Antibody [PSH17-99]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human ATF3 aa 1-100.
种属反应性
Human, Mouse
验证应用
WB
靶点分子量
Predicted band size: 21 kDa
阳性对照
HEK-293 cell lysate, THP-1 treated with 80nM TPA for 24 hours then treated with 1μg/mL LPS for 15 minutes cell lysate, RAW264.7 treated with 1μg/mL LPS for 24 hours cell lysate.
偶联
unconjugated
克隆号
PSH17-99
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| WB | IHC-P | FC | IF-Cell | |
|---|---|---|---|---|
| human |
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| mouse |
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| rat |
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产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
靶点
功能
Cyclic AMP-dependent transcription factor ATF-3 is a protein that, in humans, is encoded by the ATF3 gene. ATF-3 is induced upon physiological stress in various tissues. It is also a marker of regeneration following injury of dorsal root ganglion neurons, as injured regenerating neurons activate this transcription factor. Functional validation studies have shown that ATF3 can promote regeneration of peripheral neurons, but is not capable of promoting regeneration of central nervous system neurons.
背景文献
1. Hu S et al. Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis. Sci Adv. 2024 Jul
2. Huo S et al. ATF3/SPI1/SLC31A1 Signaling Promotes Cuproptosis Induced by Advanced Glycosylation End Products in Diabetic Myocardial Injury. Int J Mol Sci. 2023 Jan
亚细胞定位
Nucleus.
别名
Activating transcription factor 3 antibody
ATF3 antibody
ATF3_HUMAN antibody
ATF3deltaZip2 antibody
ATF3deltaZip2c antibody
ATF3deltaZip3 antibody
cAMP dependent transcription factor ATF3 antibody
cAMP-dependent transcription factor ATF-3 antibody
Cyclic AMP dependent transcription factor ATF3 antibody
Cyclic AMP-dependent transcription factor ATF-3 antibody
图片
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☑ Relative expression (RE),Cell treatment (CT)
Western blot analysis of ATF3 on different lysates with Rabbit anti-ATF3 antibody (HA723964) at 1/5,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: MCF7 cell lysate (negative)
Lane 3: THP-1 cell lysate
Lane 4: THP-1 treated with 80nM TPA for 24 hours then treated with 1μg/mL LPS for 15 minutes cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: RAW264.7 treated with 1μg/mL LPS for 24 hours cell lysate
Lane 7: NIH/3T3 cell lysate (negative)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21/19 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723964) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
引文
-
AND-1 Enhances Erastin-Induced Ferroptosis in Ovarian Cancer Cells by Down-Regulating SLC7A11 and NRF2
期刊: Biofactors
DOI: 10.1002/biof.70117
IF: 5.2
应用: WB
反应种属: Human
发表时间: 2026 May
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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