AFP Rabbit Polyclonal Antibody
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Catalog# R1601-1
AFP Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Rat
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Mouse
预测的种属支持售后
概述
产品名称
AFP Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within N-terminal human AFP.
种属反应性
Human, Rat (Predicted: Mouse)
预测的种属支持售后
验证应用
WB, IF-Cell, IHC-P, FC
分子量
Predicted band size: 69 kDa
阳性对照
MCF-7 cell lysate, PC-12 cell lysate, human liver tissue lysate, HepG2, MCF-7, Hela, human liver cancer tissue, human breast cancer tissue, 293T.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:100
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FC
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1:50-1:100
靶点
功能
α-fetoprotein (AFP) is expressed in fetal liver at varying levels throughout development and is present only in trace amounts in normal adult tissues. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker. AFP binds copper, nickel and fatty acids, and in some cases may bind serum albumin or estrogen. It has been demonstrated that the AFP promoter is a target for NF-1 (nuclear factor-1), HNF-1 (hepatocyte nuclear factor-1) and C/EBP transcription factors. While HNF-1 binding to the AFP promoter results in AFP expression, NF-1 binding results in a decrease in AFP promoter activity.
背景文献
暂无
序列相似性
Belongs to the ALB/AFP/VDB family.
组织特异性
Plasma. Synthesized by the fetal liver and yolk sac.
翻译后修饰
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.; Sulfated.
亚细胞定位
Secreted.
别名
Afp antibody
AFPD antibody
Alpha fetoglobulin antibody
Alpha fetoprotein antibody
Alpha fetoprotein precursor antibody
Alpha-1-fetoprotein antibody
Alpha-fetoglobulin antibody
Alpha-fetoprotein antibody
alpha-fetoprotein, Hereditary persistence of, included antibody
FETA antibody
展开图片
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Western blot analysis of AFP on different lysates using anti-AFP antibody at 1/1,000 dilution.
Postive control:
Lane 1: MCF-7 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Human liver tissue lysate -
ICC staining AFP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining AFP in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining AFP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of 239T cells with AFP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
AFP Recombinant Rabbit Monoclonal Antibody [PD00-71]
Application: IHC-P,WB
Reactivity: Human
Conjugate: unconjugated
同靶点 & 同通路的产品
AFP Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
