AFP Recombinant Rabbit Monoclonal Antibody [PD00-71]
Catalog# HA721238
AFP Recombinant Rabbit Monoclonal Antibody [PD00-71]
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IHC-P
-
WB
-
Human
概述
产品名称
AFP Recombinant Rabbit Monoclonal Antibody [PD00-71]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant full length protein corresponding to Human AFP.
种属反应性
Human
验证应用
IHC-P, WB
分子量
Predicted band size: 69 kDa
阳性对照
Human placenta tissue, human poorly differentiated hepatocellular carcinoma tissue, HepG2 cell lysates.
偶联
unconjugated
克隆号
PD00-71
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-P
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1:200
-
WB
-
1:1,000
靶点
功能
Alpha-fetoprotein (AFP) is the most abundant plasma protein found in the human fetus. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms. Alpha-Fetoprotein (AFP) is synthesized by the cells of the embryonic yolk sac, fetal liver and fetal intestinal tract. This secretory protein is synthesized primarily in the fetal liver whereas expression is repressed in adult liver. Anti-AFP has been immunohistochemically demonstrated in hepatocellular carcinoma (HCC) and shows no immunoreactivity in normal liver. AFP levels decrease soon after birth. In abnormal tissues, expression of AFP has been demonstrated in hepatocellular carcinoma, hepatoid adenocarcinoma, germ cell tumors and particularly yolk sac tumor. The anti-AFP antibody may be useful for the identification of neoplastic liver diseases, yolk sac tumors and mixed germ cell tumors.
背景文献
1. Mizejewski GJ. Alpha-fetoprotein structure and function: relevance to isoforms, epitopes, and conformational variants. Exp Biol Med. 2001; 226: 377-408.
2. Lazarevich NL. Molecular mechanisms of alpha-fetoprotein gene expression. Biochemistry (Mosc). 2000; 65:117-33.
亚细胞定位
Secreted
UNIPROT
别名
Afp antibody
AFPD antibody
Alpha fetoglobulin antibody
Alpha fetoprotein antibody
Alpha fetoprotein precursor antibody
Alpha-1-fetoprotein antibody
Alpha-fetoglobulin antibody
Alpha-fetoprotein antibody
alpha-fetoprotein, Hereditary persistence of, included antibody
FETA antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-AFP antibody (HA721238) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721238) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human poorly differentiated hepatocellular carcinoma tissue with Rabbit anti-AFP antibody (HA721238) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721238) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human well-differentiated hepatocellular carcinoma tissue (negative) with Rabbit anti-AFP antibody (HA721238) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721238) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of AFP on HepG2 cell lysates with Rabbit anti-AFP antibody (HA721238) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721238) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"