DNA Ligase IV Recombinant Rabbit Monoclonal Antibody [JM64-32]
Catalog# ET1705-77
DNA Ligase IV Recombinant Rabbit Monoclonal Antibody [JM64-32]
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WB
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IHC-P
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IF-Cell
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Human
概述
产品名称
DNA Ligase IV Recombinant Rabbit Monoclonal Antibody [JM64-32]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human DNA Ligase IV aa 484-705 / 911.
种属反应性
Human
验证应用
WB, IHC-P, IF-Cell
分子量
Predicted band size: 104 kDa
阳性对照
HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, human colon carcinoma tissue, human placenta tissue.
偶联
unconjugated
克隆号
JM64-32
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
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IF-Cell
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1:10-1:50
靶点
功能
The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA. These stories form a critical complex composed of two molecules of each protein that preferentially bind DNA with nicks or broken ends. As an obligatory accessory molecule, XRCC4 binds to DNA Ligase IV and enhances its joining activity. The XRCC4 / DNA Ligase IV complex is also involved in V (D) J recombination. V (D) (in) a pair of high incidence of apoptosis in the development nervous system and a block in B and T cell maturation.
背景文献
1. Rasmussen RD et al. Enhanced efficacy of combined HDAC and PARP targeting in glioblastoma. Mol Oncol 10(5):751-63 (2016).
2. Morgenroth A et al. Hedgehog signaling sensitizes glioma stem cells to endogenous nano-irradiation. Oncotarget 5:5483-93 (2014).
序列相似性
Belongs to the ATP-dependent DNA ligase family.
组织特异性
Testis, thymus, prostate and heart.
亚细胞定位
Nucleus.
UNIPROT
别名
DNA ligase IV ATP dependent antibody
DNA joinase antibody
DNA ligase 4 antibody
DNA ligase IV antibody
DNA repair enzyme antibody
DNLI4_HUMAN antibody
LIG 4 antibody
LIG4 antibody
LIG4S antibody
Ligase IV antibody
展开图片
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Western blot analysis of DNA Ligase IV on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-DNA Ligase IV antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-77, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DNA Ligase IV antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-77, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"