DOPA Decarboxylase Recombinant Rabbit Monoclonal Antibody [JA53-16]
Catalog# ET1704-94
DOPA Decarboxylase Recombinant Rabbit Monoclonal Antibody [JA53-16]
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
预测的种属支持售后
概述
产品名称
DOPA Decarboxylase Recombinant Rabbit Monoclonal Antibody [JA53-16]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human DOPA Decarboxylase aa 27-76 / 480.
种属反应性
Human, Mouse (Predicted: Rat)
预测的种属支持售后
验证应用
WB, IHC-P
分子量
Predicted band size: 54 kDa
阳性对照
Human kidney tissue, human liver tissue, mouse liver tissue lysates, HepG2 cell lysates.
偶联
unconjugated
克隆号
JA53-16
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-1:1,000
-
IHC-P
-
1:50-1:400
靶点
功能
DOPA decarboxylase (DDC), also designated aromatic-L-amino-acid decarboxylase (AADC) belongs to the group II decarboxylase family of proteins. DDC, which can form a homodimer, is an important protein in the catecholamine biosynthesis pathway. DDC acts as a catalyst in the decarboxylation of L-5-hydroxytryptophan to serotonin, L-3,4-dihydroxyphenylalanine (DOPA) to dopamine and L-tryptophan to tryptamine. Defects in the gene encoding for DDC may cause the autosomal recessive disorder AADC deficiency. AADC deficiency is an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency. This causes poor feeding, psychomotor and developmental delays, lethargy, ptosis, gastrointestinal disturbances and hypothermia.
背景文献
1. Wienecke J et al. Spinal cord injury enables aromatic L-amino acid decarboxylase cells to synthesize monoamines. J Neurosci 34:11984-2000 (2014).
2. Panayotis N et al. Morphological and functional alterations in the substantia nigra pars compacta of the Mecp2-null mouse. Neurobiol Dis 41:385-97 (2011).
序列相似性
Belongs to the group II decarboxylase family.
亚细胞定位
Cytoplasm.
别名
AADC antibody
Aromatic L Amino Acid Decarboxylase antibody
Aromatic-L-amino-acid decarboxylase antibody
Ddc antibody
DDC_HUMAN antibody
DOPA decarboxylase (aromatic L-amino acid decarboxylase) antibody
DOPA decarboxylase antibody
图片
-
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DOPA Decarboxylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-94, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-DOPA Decarboxylase antibody (ET1704-94) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-94) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of DOPA Decarboxylase on mouse liver tissue lysates with Rabbit anti-DOPA Decarboxylase antibody (ET1704-94) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-94) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of DOPA Decarboxylase on HepG2 cell lysates with Rabbit anti-DOPA Decarboxylase antibody (ET1704-94) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 55 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-94) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"