CCNT1 Rabbit Polyclonal Antibody
Catalog# HA500313
CCNT1 Rabbit Polyclonal Antibody
-
WB
-
Human
概述
产品名称
CCNT1 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within human CCNT1 aa 275-535 / 726.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 81 kDa
阳性对照
K562 cell lysate, Hela cell lysate, HL-60 cell lysates.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:500-1:1,000
靶点
功能
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II).(Microbial infection) In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
背景文献
1. Jang M.K., et al. The bromodomain protein Brd4 is a positive regulatory component of P-TEFb and stimulates RNA polymerase II-dependent transcription. Mol. Cell 19:523-534(2005).
2. Yang Z., et al. Recruitment of P-TEFb for stimulation of transcriptional elongation by the bromodomain protein Brd4. Mol. Cell 19:535-545(2005).
亚细胞定位
Nucleus.
UNIPROT
别名
CCN T1 antibody
CCNT antibody
CCNT 1 antibody
Ccnt1 antibody
CCNT1_HUMAN antibody
CDK9 associated C type protein antibody
Cyc T1 antibody
Cyclin C related protein antibody
Cyclin T antibody
cyclin T1 antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of CCNT1 on different lysates with Rabbit anti-CCNT1 antibody (HA500313) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CCNT1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 81 kDa
Observed band size: 81 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500313) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CCNT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500313, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: Hela cell lysate -
Western blot analysis of CCNT1 on HL-60 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500313, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"