BTNL3 Rabbit Polyclonal Antibody
Catalog# HA500238
BTNL3 Rabbit Polyclonal Antibody
-
WB
-
Human
概述
产品名称
BTNL3 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human BTNL3 aa 440-450.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 52 kDa
阳性对照
HL-60 cell lysate, human small intestine tissue lysate.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:500
靶点
功能
Butyrophilin-like 3 (BTNL3) is a member of the BTN/MOG Ig-superfamily which is coded by the BTNL3 gene, also known as COLF4100. BTNL3 is a 466 amino acid (aa) type I transmembrane protein that contains a 220 aa extracellular domain (ECD), a 21 aa helical transmembrane domain, and a 208 aa cytoplasmic domain. The ECD features both an IgV and IgC domain, which is commonplace in BTN/BTNL proteins, but the IgC domain is relatively weak. The ECD of human BTNL3 shares only 37% and 34% aa sequence identity with mouse (referred to as BTNL1) and rat, respectively. Human BTNL3 mRNA has been identified in many tissues including small intestine, colon, liver, lung, bone marrow and spleen tissue, and it is highly expressed in neutrophils. BTNL3 expression has also been found to be down-regulated in colon cancer tumors. The specific function of BTNL3, however, has yet to be fully elucidated. R&D in house data indicate that BTNL3 protein inhibits the secretion of IFN-gamma from anti-CD3 activated human T cells.
背景文献
1. Jandke A. et. al. Butyrophilin-like proteins display combinatorial diversity in selecting and maintaining signature intraepithelial gammadelta T cell compartments. Nat Commun. 2020 Jul
2. Willcox CR. et. al. Butyrophilin-like 3 Directly Binds a Human Vgamma4(+) T Cell Receptor Using a Modality Distinct from Clonally-Restricted Antigen. Immunity. 2019 Nov
亚细胞定位
Membrane
UNIPROT
别名
BTNL3 antibody
BTNL3_HUMAN antibody
BTNLR antibody
Butyrophilin like protein 3 antibody
Butyrophilin like receptor antibody
Butyrophilin-like protein 3 antibody
Butyrophilin-like receptor antibody
COLF4100 antibody
图片
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Western blot analysis of BTNL3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500238, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HL-60 cell lysate
Lane 2: Human small intestine tissue lysate
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"