概述
产品名称
DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide immune sequence is CDYKDDDDK.
种属反应性
Species independent
验证应用
WB, IP
阳性对照
DYKDDDDK Tag recombinant protein, N-terminal FLAG-tagged recombinant protein, C-terminal FLAG-tagged recombinant protein.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:5,000
-
IP
-
1:10-1:100
发表文章中的应用
WB | 查看 37 篇文献如下 |
IP | 查看 7 篇文献如下 |
ChIP | 查看 2 篇文献如下 |
发表文章中的种属
Human | 查看 25 篇文献如下 |
Mouse | 查看 8 篇文献如下 |
pig | 查看 1 篇文献如下 |
靶点
功能
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.
背景文献
暂无
别名
DDDDK epitope tag antibody
DDDK antibody
ddk antibody
DYKDDDDK antibody
DYKDDDDK epitope tag antibody
DYKDDDDK tag antibody
ECS epitope tag antibody
ECS tag antibody
Enterokinase Cleavage Site epitope tag antibody
Enterokinase Cleavage Site tag antibody
展开DDDDK epitope tag antibody
DDDK antibody
ddk antibody
DYKDDDDK antibody
DYKDDDDK epitope tag antibody
DYKDDDDK tag antibody
ECS epitope tag antibody
ECS tag antibody
Enterokinase Cleavage Site epitope tag antibody
Enterokinase Cleavage Site tag antibody
FLAG antibody
FLAG tag antibody
折叠图片
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Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: N-terminal FLAG-tagged recombinant protein
Lane 2: C-terminal FLAG-tagged recombinant protein
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Ubiquitination-dependent degradation of DHX36 mediated by porcine circovirus type 3 capsid protein
Author: Jie Zhao, Qianhong Dai, Haoyu Sun, Beiyi Zhou, Xiaoyuan Lan, Yonghui Qiu, Qianqian Zhang, Dedong Wang, Yongqiu Cui, Jinshuo Guo, Lei Hou, Jue Liu, Jianwei Zhou
PMID: 39862752
应用: WB
反应种属: Human,Porcine
发表时间: 2025 Jan
-
Citation
-
Hsa_circ_0000825 promotes the progression of laryngeal squamous cell carcinoma by sponging miR-766 and interacting with ELAVL1
Author: Yu Miaomiao,et al
PMID: NO PMID20240904
应用: RIP
反应种属:
发表时间: 2024 Sep
-
Citation
-
RSAD2 suppresses viral replication by interacting with the Senecavirus A 2 C protein
Author: Lei Hou,et al
PMID: NO PMID 2024101211
应用: WB
反应种属: Human
发表时间: 2024 Oct
-
Citation
-
Activation of the FOXM1/ASF1B/PRDX3 axis confers hyperproliferative and antioxidative stress reactivity to gastric cancer
Author: Zhou Zhao,et al
PMID: 38537775
应用: ChIP
反应种属: Human
发表时间: 2024 Mar
-
Citation
-
FAM188B promotes the growth, metastasis, and invasion of hepatocellular carcinoma by targeting the HRNPA1/PKM2 axis
Author: Mu Mingshan,et al
PMID: 38844182
应用: WB
反应种属: Human
发表时间: 2024 Jun
-
Citation
-
Linear ubiquitination regulates the KSHV replication and transcription activator protein to control infection
Author: Luan Yi,et al
PMID: 38951495
应用: WB
反应种属: Human
发表时间: 2024 Jul
-
Citation
-
Taxus chinensis var. mairei (Lemée et Lévl) Cheng et L.K. Fu overcomes the resistance to osimertinib in EGFR-mutant non-small-cell lung cancer via suppression of ERK1/2-related cholesterol biosynthesis
Author: Dai Shuying,et al
PMID: 39032664
应用: WB
反应种属:
发表时间: 2024 Jul
-
Citation
-
Claudin-18.2 mediated interaction of gastric Cancer cells and Cancer-associated fibroblasts drives tumor progression
Author: Liu Shengde,et al
PMID: 38200591
应用: WB
反应种属: Human,Mouse
发表时间: 2024 Jan
-
Citation
-
BZW2 promotes malignant progression in lung adenocarcinoma through enhancing the ubiquitination and degradation of GSK3β
Author: Jin Kai,et al
PMID: 38424042
应用: WB,IP
反应种属: Human
发表时间: 2024 Feb
-
Citation
-
Protein kinase Cdc7 supports viral replication by phosphorylating Avibirnavirus VP3 protein
Author: Deng T, Du L, Ding S, et al
PMID: 37902398
应用: WB
反应种属: Human
发表时间: 2023 Oct
-
Citation
-
Acetyl-coenzyme A acetyltransferase 1 promotes brown adipogenesis by activating the AMPK-PGC1α signaling pathway
Author:
PMID: 37582428
应用: WB
反应种属: Mouse
发表时间: 2023 Oct
-
Citation
-
Discovery of a Selective and Orally Bioavailable FGFR2 Degrader for Treating Gastric Cancer
Author: Ma, L., Li, Y., Luo, R., Wang, Y., Cao, J., Fu, W., Qian, B., Zheng, L., Tang, L., Lv, X., Zheng, L., Liang, G., & Chen, L.
PMID: 37220310
应用: WB
反应种属: Mouse,Human
发表时间: 2023 Jun
-
Citation
-
ARIH1 activates STING-mediated T-cell activation and sensitizes tumors to immune checkpoint blockade
Author:
PMID: 37429863
应用: WB
反应种属: Human
发表时间: 2023 Jul
-
Citation
-
Transforming acidic coiled-coil containing protein 3 suppresses influenza A virus replication by impeding viral endosomal trafficking and nuclear import
Author:
PMID: 37148621
应用: IF-cell
反应种属:
发表时间: 2023 Jul
-
Citation
-
JWA inhibits nicotine-induced lung cancer stemness and progression through CHRNA5/AKT-mediated JWA/SP1/CD44 axis
Author: jwzhou@njmu.edu.cn
PMID: 37224781
应用: WB
反应种属: Mouse
发表时间: 2023 Jul
-
Citation
-
(Pro)renin receptor promotes colorectal cancer progression through inhibiting the NEDD4L-mediated Wnt3 ubiquitination and modulating gut microbiota
Author: Wang, J., Ding, Y., Li, D., Zhu, N., Nishiyama, A., & Yuan, Y.
PMID: 36597142
应用: WB
反应种属: Human
发表时间: 2023 Jan
-
Citation
-
Heterogeneous Nuclear Protein U Degraded the m6A Methylated TRAF3 Transcript by YTHDF2 To Promote Porcine Epidemic Diarrhea Virus Replication
Author: Zhou H, Yan Y, Gao J, et al
PMID: 36752613
应用: WB,IP
反应种属: Human
发表时间: 2023 Feb
-
Citation
-
TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus
Author: Zhao, L., Liu, Q., Huang, J., Lu, Y., Zhao, Y., & Ping, J.
PMID: 36084138
应用: WB
反应种属: Human
发表时间: 2022 Sept
-
Citation
-
The Network of Interactions between the Porcine Epidemic Diarrhea Virus Nucleocapsid and Host Cellular Proteins
Author: Zhou, J., Qiu, Y., Zhao, J., Wang, Y., Zhu, N., Wang, D., Cui, Y., Guo, J., Sun, T., Ji, Y., Wu, Z., Zeng, P., Li, J., Feng, X., Hou, L., & Liu, J.
PMID: 36298827
应用: WB
反应种属: Pig
发表时间: 2022 Oct
-
Citation
-
Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins
Author: Zhou, J., Wang, Y., Zhou, L., Qiu, Y., Zhao, J., Dai, B., Feng, X., Hou, L., & Liu, J.
PMID: 35632681
应用: WB
反应种属: Human
发表时间: 2022 May
-
Citation
-
TRAF6 autophagic degradation by avibirnavirus VP3 inhibits antiviral innate immunity via blocking NFKB/NF-κB activation
Author: Deng, T., Hu, B., Wang, X., Ding, S., Lin, L., Yan, Y., Peng, X., Zheng, X., Liao, M., Jin, Y., Dong, W., Gu, J., & Zhou, J.
PMID: 35266845
应用: WB
反应种属: Chicken
发表时间: 2022 Mar
-
Citation
-
CDK1 Promotes Epithelial-Mesenchymal Transition and Migration of Head and Neck Squamous Carcinoma Cells by Repressing ∆Np63α-Mediated Transcriptional Regulation
Author: Chen, H., Hu, K., Xie, Y., Qi, Y., Li, W., He, Y., Fan, S., Liu, W., & Li, C.
PMID: 35806389
应用: WB
反应种属:
发表时间: 2022 Jul
-
Citation
-
BmNPV p35 Reduces the Accumulation of Virus-Derived siRNAs and Hinders the Function of siRNAs to Facilitate Viral Infection
Author: Zhao, S., Chen, G., Kong, X., Chen, N., & Wu, X.
PMID: 35251046
应用:
反应种属:
发表时间: 2022 Feb
-
Citation
-
Contribution of DEAD-Box RNA Helicase 21 to the Nucleolar Localization of Porcine Circovirus Type 4 Capsid Protein
Author: Zhou, J., Wang, Y., Qiu, Y., Wang, Y., Yang, X., Liu, C., Shi, Y., Feng, X., Hou, L., & Liu, J.
PMID: 35283818
应用: WB
反应种属: Human
发表时间: 2022 Feb
-
Citation
-
The Nucleolar Localization Signal of Porcine Circovirus Type 4 Capsid Protein Is Essential for Interaction With Serine-48 Residue of Nucleolar Phosphoprotein Nucleophosmin-1
Author: Zhou, J., Qiu, Y., Zhu, N., Zhou, L., Dai, B., Feng, X., Hou, L., & Liu, J.
PMID: 34745055
应用: IP
反应种属:
发表时间: 2021 Oct
-
Citation
-
<html>Genome-Wide Identification Reveals That <i>Nicotiana benthamiana</i> Hypersensitive Response (HR)-Like Lesion Inducing Protein 4 (NbHRLI4) Mediates Cell Death and Salicylic Acid-Dependent Defense Responses to Turnip Mosaic Virus. Frontiers in plant science, 12, 627315.</html>
Author: Wu, X., Lai, Y., Rao, S., Lv, L., Ji, M., Han, K., Weng, J., Lu, Y., Peng, J., Lin, L., Wu, G., Chen, J., Yan, F., & Zheng, H.
PMID: 34113359
应用: WB
反应种属: Nicotiana tabacum
发表时间: 2021 May
-
Citation
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<html>Inhibition of Antiviral Innate Immunity by <i>Avibirnavirus</i> VP3 via Blocking TBK1-TRAF3 Complex Formation and IRF3 Activation. mSystems, 6(3), e00016-21.</html>
Author: Deng, T., Hu, B., Wang, X., Lin, L., Zhou, J., Xu, Y., Yan, Y., Zheng, X., & Zhou, J.
PMID: 33975961
应用: WB
反应种属: Human
发表时间: 2021 May
-
Citation
-
Label‐free proteomic dissection on dptP‐deletion mutant uncovers dptP involvement in strain growth and daptomycin tolerance of Streptomyces roseosporus
Author:
PMID: 33369164
应用: WB
反应种属:
发表时间: 2021 Mar
-
Citation
-
Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB
Author:
PMID: 33553178
应用: WB
反应种属: Human
发表时间: 2021 Jan
-
Citation
-
The serine-48 residue of nucleolar phosphoprotein nucleophosmin-1 plays critical role in subcellular localization and interaction with porcine circovirus type 3 capsid protein. Veterinary research, 52(1), 4.
Author: Zhou, J., Li, J., Li, H., Zhang, Y., Dong, W., Jin, Y., Yan, Y., Gu, J., & Zhou, J.
PMID: 33413620
应用: WB
反应种属:
发表时间: 2021 Jan
-
Citation
-
DeSUMOylation of Apoptosis Inhibitor 5 by Avibirnavirus VP3 Supports Virus Replication
Author: Deng, T., Hu, B., Wang, X., Yan, Y., Zhou, J., Lin, L., Xu, Y., Zheng, X., & Zhou, J.
PMID: 34372697
应用: WB
反应种属: Human
发表时间: 2021 Aug
-
Citation
-
ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation. Nature communications, 12(1), 2346.
Author: Wu, Y., Zhang, C., Liu, X., He, Z., Shan, B., Zeng, Q., Zhao, Q., Zhu, H., Liao, H., Cen, X., Xu, X., Zhang, M., Hou, T., Wang, Z., Yan, H., Yang, S., Sun, Y., Chen, Y., Wu, R., Xie, T., … Xia, H.
PMID: 33879767
应用: WB
反应种属: Human
发表时间: 2021 Apr
-
Citation
-
Features of Nuclear Export Signals of NS2 Protein of Influenza D Virus
Author: Jihui Ping
PMID: 33003329
应用: COIP
反应种属: Human
发表时间: 2020 Sep
-
Citation
-
Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation
Author: Di Wang
PMID: 32937100
应用: WB,IP
反应种属: Mouse
发表时间: 2020 Oct
-
Citation
-
The RCAN1.4-calcineurin/NFAT signaling pathway is essential for hypoxic adaption of intervertebral discs
Author: Jian Chen;Fengdong Zhao
PMID: 32467610
应用: WB,IP
反应种属: Human
发表时间: 2020 May
-
Citation
-
Protein Interactions Network of Porcine Circovirus Type 2 Capsid With Host Proteins
Author: Jiyong Zhou
PMID: 32582087
应用: WB
反应种属: pig
发表时间: 2020 Jun
-
Citation
-
Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function
Author: Guoqiang Xu;Xiangao Huang
PMID: 33392195
应用: IP
反应种属: Human
发表时间: 2020 Dec
-
Citation
-
Nucleolar protein NPM1 is essential for circovirus replication by binding to viral capsid
Author: Jinyan Gu; Jiyong Zhou
PMID: 33073687
应用: WB,CoIP
反应种属: Human
发表时间: 2020 Dec
-
Citation
-
Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress
Author: Wei Liu;Chun Tang
PMID: 31857589
应用: IP,CO-IP
反应种属: Human
发表时间: 2019 Dec
-
Citation
-
Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and StressActivated Protein Kinase 1-Dependent Manner
Author: Qin Liu
PMID: 30396897
应用: WB
反应种属: Escherichia coli
发表时间: 2019 Dec
-
Citation
-
TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction
Author: Wei Liu
PMID: 30767704
应用: WB
反应种属: HEK293 cells
发表时间: 2019 Aug
-
Citation
-
Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages
Author: 王迪
PMID: 30366764
应用: WB
反应种属: None
发表时间: 2018 Nov
-
Citation
-
E4bp4 Regulates Carboxylesterase 2 Enzymes through Repression of the Nuclear Receptor Rev-erbα in Mice
Author: Baojian Wu
PMID: 29653076
应用: WB
反应种属: Mouse
发表时间: 2018 Jun
-
Citation
-
Downregulated NDR1 protein kinase inhibits innate immune response by initiating an miR146a-STAT1 feedback loop
Author: Jihong Sun6 & Xiaojian Wang1
PMID: 30018336
应用: WB
反应种属: Mouse
发表时间: 2018 Jul
-
Citation
-
Dicer interacts with SIRT7 and regulates H3K18 deacetylation in response to DNA damaging agents
Author: Kaifu T
PMID: 26704979
应用: WB
反应种属: Human
发表时间: 2016 May
-
Citation
-
MSX3 switches microglia polarization and protects from inflammation-induced demyelination
Author:
PMID: 25904788
应用: ChIP
反应种属: Mouse
发表时间: 2015 Apr
-
Citation
