DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody
Catalog# 0912-1
DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody
-
WB
-
IP
-
Species independent
-
unconjugated
概述
产品名称
DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide immune sequence is CDYKDDDDK.
种属反应性
Species independent
验证应用
WB, IP
阳性对照
DYKDDDDK Tag recombinant protein, N-terminal FLAG-tagged recombinant protein, C-terminal FLAG-tagged recombinant protein.
偶联
unconjugated
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:5,000
-
IP
-
1-2μg/sample
靶点
功能
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.
背景文献
暂无
别名
DDDDK epitope tag antibody
DDDK antibody
ddk antibody
DYKDDDDK antibody
DYKDDDDK epitope tag antibody
DYKDDDDK tag antibody
ECS epitope tag antibody
ECS tag antibody
Enterokinase Cleavage Site epitope tag antibody
Enterokinase Cleavage Site tag antibody
展开DDDDK epitope tag antibody
DDDK antibody
ddk antibody
DYKDDDDK antibody
DYKDDDDK epitope tag antibody
DYKDDDDK tag antibody
ECS epitope tag antibody
ECS tag antibody
Enterokinase Cleavage Site epitope tag antibody
Enterokinase Cleavage Site tag antibody
FLAG antibody
FLAG tag antibody
折叠图片
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Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: N-terminal FLAG-tagged recombinant protein
Lane 2: C-terminal FLAG-tagged recombinant protein -
DYKDDDDK Tag (FLAG) was immunoprecipitated from 0.2 mg L-929 transfected with FLAG-tagged CD5 cell lysate with 0912-1 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using 0912-1 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: L-929 transfected with FLAG-tagged CD5 cell lysate (input)
Lane 2: 0912-1 IP in L-929 transfected with FLAG-tagged CD5 cell lysate
Lane 3: Rabbit IgG instead of 0912-1 in L-929 transfected with FLAG-tagged CD5 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Covalent modification of Keap1 Cys489 by NU6300 activates Nrf2 signaling and suppresses NLRP3 inflammasome-mediated pyroptosis
期刊: Journal Of Pharmaceutical Analysis
DOI: 10.1016/j.jpha.2025.101458
IF: 8.9
应用: WB,IP,CoIP
反应种属: Human
发表时间: 2025 Sept
-
Med20 regulates myelination in the peripheral nervous system by modulating ferroptosis of Schwann cells
期刊: Cell Reports
DOI: 10.1016/j.celrep.2025.116448
IF: 6.9
应用: IP,CoIP,ChIP
反应种属: Rat
发表时间: 2025 Oct
-
DEAD-box RNA helicase 10 inhibits porcine circovirus type 3 replication by interacting with the viral capsid protein and activating interferon responses
期刊: Journal Of Virology
DOI: 10.1128/jvi.00576-25
IF: 4
应用: WB
反应种属: Human,Pig
发表时间: 2025 May
-
Mutagenesis of AcSQBP9 in kiwifruit results in reduction of malate via alteration of the expression of a plastidial malate dehydrogenase
期刊: Plant Journal
DOI: 10.1111/tpj.70082
IF: 6.2
应用: WB
反应种属: Kiwifruit
发表时间: 2025 Mar
-
API5 Phosphorylation Promotes Antiviral Immunity by Inhibiting Degradation of Cytosolic RNA Sensor RLRs
期刊: Advanced Science
DOI: 10.1002/advs.202505479
IF: 14.1
应用: WB
反应种属: Human
发表时间: 2025 Jul
-
CDK8 mediated inflammatory microenvironment aggravates osteoarthritis progression
期刊: Journal Of Advanced Research
DOI: 10.1016/j.jare.2025.01.017
IF: 13
应用: WB
反应种属: Human
发表时间: 2025 Jan
-
Ubiquitination-dependent degradation of DHX36 mediated by porcine circovirus type 3 capsid protein
期刊: VIROLOGY
DOI: 10.1016/j.virol.2025.110419
IF: 2.4
应用: WB
反应种属: Human,Porcine
发表时间: 2025 Jan
-
Ubiquitination-dependent degradation of DHX36 mediated by porcine circovirus type 3 capsid protein
期刊: Virology
DOI:
IF: 2.8
应用: WB
反应种属: Human,Pig
发表时间: 2025 Jan
-
HnRNPC triggers the degradation of MITA to suppress the interferon-mediated antiviral response
期刊: Veterinary Research
DOI: 10.1186/s13567-025-01463-6
IF: 3.7
应用: WB
反应种属: Human,Fish
发表时间: 2025 Feb
-
In vivo chemogenetic RNA editing of macrophages by bioengineered viruses for sepsis treatment
期刊: Nature Communications
DOI: 10.1038/s41467-025-67655-y
IF: 15.7
应用: WB
反应种属: Mouse
发表时间: 2025 Dec
-
Heat shock protein A1L restricts influenza A virus by ubiquitination of NA
期刊: Journal Of Virology
DOI: 10.1128/jvi.00771-25
IF: 3.8
应用: WB
反应种属: Human
发表时间: 2025 Aug
-
NtPP2C42 Negatively Regulates NtCLE3-NtBAM3-3 Mediated Drought Tolerance in Tobacco
期刊: Plant Physiology And Biochemistry
DOI: 10.1016/j.plaphy.2025.110378
IF: 5.7
应用: WB
反应种属: Nicotiana benthamiana
发表时间: 2025 Aug
-
PDZK1 inhibits MRP2-mediated oxaliplatin chemosensitivity in hepatocellular carcinoma
期刊: Scientific Reports
DOI: 10.1038/s41598-025-98085-x
IF: 3.8
应用: IF
反应种属: Human
发表时间: 2025 Apr
-
Staphylococcus aureus reprograms CASP8 (caspase 8) signaling to evade cell death and Xenophagy
期刊: Autophagy
DOI: 10.1080/15548627.2025.2483887
IF: 14.6
应用: WB
反应种属: Cow
发表时间: 2025 Apr
-
Stearoyl coenzyme A desaturase 1 (SCD1) regulates foot-and-mouth disease virus replication by modulating host cell lipid metabolism and viral protein 2C-mediated replication complex formation
期刊: Journal Of Virology
DOI: 10.1128/jvi.00902-24
IF: 4
应用: WB
反应种属: Species independent
发表时间: 2024 Sept
-
Hsa_circ_0000825 promotes the progression of laryngeal squamous cell carcinoma by sponging miR-766 and interacting with ELAVL1
期刊: Heliyon
DOI:
IF: 3.7
应用: RIP
反应种属: Species independent
发表时间: 2024 Sep
-
RSAD2 suppresses viral replication by interacting with the Senecavirus A 2 C protein
期刊: Veterinary Research
DOI:
IF: 3.7
应用: WB
反应种属: Human
发表时间: 2024 Oct
-
Seneca valley virus 3C protease blocks EphA2-Mediated mTOR activation to facilitate viral replication
期刊: Microbial Pathogenesis
DOI: 10.1016/j.micpath.2024.106673
IF: 3.3
应用: WB
反应种属: Species independent
发表时间: 2024 May
-
Activation of the FOXM1/ASF1B/PRDX3 axis confers hyperproliferative and antioxidative stress reactivity to gastric cancer
期刊: Cancer Letters
DOI:
IF: 9.7
应用: ChIP
反应种属: Human
发表时间: 2024 Mar
-
FAM188B promotes the growth, metastasis, and invasion of hepatocellular carcinoma by targeting the HRNPA1/PKM2 axis
期刊: Biochimica Et Biophysica Acta - Molecular Cell Research
DOI:
IF: 5.1
应用: WB
反应种属: Human
发表时间: 2024 Jun
-
Taxus chinensis var. mairei (Lemée et Lévl) Cheng et L.K. Fu overcomes the resistance to osimertinib in EGFR-mutant non-small-cell lung cancer via suppression of ERK1/2-related cholesterol biosynthesis
期刊: Journal Of Ethnopharmacology
DOI:
IF: 4.8
应用: WB
反应种属: Species independent
发表时间: 2024 Jul
-
Linear ubiquitination regulates the KSHV replication and transcription activator protein to control infection
期刊: Nature Communications
DOI:
IF: 14.7
应用: WB
反应种属: Human
发表时间: 2024 Jul
-
Claudin-18.2 mediated interaction of gastric Cancer cells and Cancer-associated fibroblasts drives tumor progression
期刊: Cell Communication And Signaling
DOI:
IF: 8.4
应用: WB
反应种属: Human,Mouse
发表时间: 2024 Jan
-
DEAD-box RNA helicase 21 interacts with porcine circovirus type 2 Cap protein and facilitates viral replication
期刊: Frontiers In Microbiology
DOI: 10.3389/fmicb.2024.1298106
IF: 4
应用: WB
反应种属: Pig
发表时间: 2024 Feb
-
BZW2 promotes malignant progression in lung adenocarcinoma through enhancing the ubiquitination and degradation of GSK3β
期刊: Cell Death Discovery
DOI:
IF: 6.1
应用: WB,IP
反应种属: Human
发表时间: 2024 Feb
-
IBSP Promotes Breast Cancer Bone Metastasis and Proliferation via BMP-SMAD Signaling Pathway
期刊: Cancer Reports
DOI: 10.1002/cnr2.2153
IF: 1.5
应用: WB
反应种属: Human
发表时间: 2024 Aug
-
SP-141 targets Trs85 to inhibit rice blast fungus infection and functions as a potential broad-spectrum antifungal agent
期刊: Plant Communications
DOI: 10.1016/j.xplc.2023.100724
IF: 10.5
应用: WB
反应种属: Escherichia coli
发表时间: 2023 Sept
-
EIF4A3-regulated hsa_circ_0001445 can inhibit the progression of laryngeal squamous cell carcinoma via hsa-miR-432-5p-dependent up-regulation of RGMA expression
期刊: Cell Cycle
DOI: 10.1080/15384101.2023.2274670
IF: 4.3
应用: RIP,WB
反应种属: Human
发表时间: 2023 Oct
-
Acetyl-coenzyme A acetyltransferase 1 promotes brown adipogenesis by activating the AMPK-PGC1α signaling pathway
期刊: Biochimica Et Biophysica Acta-Molecular And Cell Biology Of Lipids
DOI:
IF: 4.8
应用: WB
反应种属: Mouse
发表时间: 2023 Oct
-
Protein kinase Cdc7 supports viral replication by phosphorylating Avibirnavirus VP3 protein
期刊: Journal Of Virology
DOI:
IF: 5.4
应用: WB
反应种属: Human
发表时间: 2023 Oct
-
Discovery of a Selective and Orally Bioavailable FGFR2 Degrader for Treating Gastric Cancer
期刊: Journal Of Medicinal Chemistry
DOI:
IF: 8.039
应用: WB
反应种属: Mouse,Human
发表时间: 2023 Jun
-
ARIH1 activates STING-mediated T-cell activation and sensitizes tumors to immune checkpoint blockade
期刊: Nature Communications
DOI:
IF: 16.6
应用: WB
反应种属: Human
发表时间: 2023 Jul
-
JWA inhibits nicotine-induced lung cancer stemness and progression through CHRNA5/AKT-mediated JWA/SP1/CD44 axis
期刊: Ecotoxicology And Environmental Safety
DOI:
IF: 6.8
应用: WB
反应种属: Mouse
发表时间: 2023 Jul
-
Transforming acidic coiled-coil containing protein 3 suppresses influenza A virus replication by impeding viral endosomal trafficking and nuclear import
期刊: Veterinary Microbiology
DOI:
IF: 3.3
应用: IF-cell
反应种属: Species independent
发表时间: 2023 Jul
-
(Pro)renin receptor promotes colorectal cancer progression through inhibiting the NEDD4L-mediated Wnt3 ubiquitination and modulating gut microbiota
期刊: Cell Communication And Signaling
DOI:
IF: 7.525
应用: WB
反应种属: Human
发表时间: 2023 Jan
-
Heterogeneous Nuclear Protein U Degraded the m6A Methylated TRAF3 Transcript by YTHDF2 To Promote Porcine Epidemic Diarrhea Virus Replication
期刊: Journal Of Virology
DOI:
IF: 5.4
应用: WB,IP
反应种属: Human
发表时间: 2023 Feb
-
TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus
期刊: PLoS Pathogens
DOI:
IF: 7.464
应用: WB
反应种属: Human
发表时间: 2022 Sept
-
The Network of Interactions between the Porcine Epidemic Diarrhea Virus Nucleocapsid and Host Cellular Proteins
期刊: Viruses
DOI:
IF: 5.818
应用: WB
反应种属: Pig
发表时间: 2022 Oct
-
Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins
期刊: Viruses
DOI:
IF: 5.048
应用: WB
反应种属: Human
发表时间: 2022 May
-
TRAF6 autophagic degradation by avibirnavirus VP3 inhibits antiviral innate immunity via blocking NFKB/NF-κB activation
期刊: Autophagy
DOI:
IF: 16.016
应用: WB
反应种属: Chicken
发表时间: 2022 Mar
-
CDK1 Promotes Epithelial-Mesenchymal Transition and Migration of Head and Neck Squamous Carcinoma Cells by Repressing ∆Np63α-Mediated Transcriptional Regulation
期刊: International Journal Of Molecular Sciences
DOI:
IF: 5.924
应用: WB
反应种属: Species independent
发表时间: 2022 Jul
-
BmNPV p35 Reduces the Accumulation of Virus-Derived siRNAs and Hinders the Function of siRNAs to Facilitate Viral Infection
期刊: Frontiers In Immunology
DOI:
IF: 7.561
应用: WB
反应种属: Species independent
发表时间: 2022 Feb
-
Contribution of DEAD-Box RNA Helicase 21 to the Nucleolar Localization of Porcine Circovirus Type 4 Capsid Protein
期刊: Contribution Of Dead-Box Rna Helicase 21 To The Nucleolar Localization Of Porcine Circovirus Type 4 Capsid Protein
DOI:
IF: 5.640
应用: WB
反应种属: Human
发表时间: 2022 Feb
-
The Nucleolar Localization Signal of Porcine Circovirus Type 4 Capsid Protein Is Essential for Interaction With Serine-48 Residue of Nucleolar Phosphoprotein Nucleophosmin-1
期刊: Frontiers In Microbiology
DOI:
IF: 5.81
应用: IP
反应种属: Species independent
发表时间: 2021 Oct
-
<html><html><html>Genome-Wide Identification Reveals That <i>Nicotiana benthamiana</i> Hypersensitive Response (HR)-Like Lesion Inducing Protein 4 (NbHRLI4) Mediates Cell Death and Salicylic Acid-Dependent Defense Responses to Turnip Mosaic Virus. Frontiers in plant science, 12, 627315.</html></html></html>
期刊: Frontiers In Plant Science
DOI:
IF: 5.753
应用: WB
反应种属: Nicotiana tabacum
发表时间: 2021 May
-
<html><html><html>Inhibition of Antiviral Innate Immunity by <i>Avibirnavirus</i> VP3 via Blocking TBK1-TRAF3 Complex Formation and IRF3 Activation. mSystems, 6(3), e00016-21.</html></html></html>
期刊: mSystems
DOI:
IF: 6.469
应用: WB
反应种属: Human
发表时间: 2021 May
-
Label‐free proteomic dissection on dptP‐deletion mutant uncovers dptP involvement in strain growth and daptomycin tolerance of Streptomyces roseosporus
期刊: Microbial Biotechnology
DOI:
IF: 5.7
应用: WB
反应种属: Species independent
发表时间: 2021 Mar
-
CDK9 Inhibitor Induces the Apoptosis of B-Cell Acute Lymphocytic Leukemia by Inhibiting c-Myc-Mediated Glycolytic Metabolism
期刊: Frontiers In Cell And Developmental Biology
DOI: 10.3389/fcell.2021.641271
IF: 6.684
应用: WB
反应种属: Human
发表时间: 2021 Mar
-
The serine-48 residue of nucleolar phosphoprotein nucleophosmin-1 plays critical role in subcellular localization and interaction with porcine circovirus type 3 capsid protein. Veterinary research, 52(1), 4.
期刊: Veterinary Research
DOI:
IF: 1.792
应用: WB
反应种属: Species independent
发表时间: 2021 Jan
-
Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB
期刊: Frontiers In Cell And Developmental Biology
DOI:
IF: 5.20
应用: WB
反应种属: Human
发表时间: 2021 Jan
-
DeSUMOylation of Apoptosis Inhibitor 5 by Avibirnavirus VP3 Supports Virus Replication
期刊: mBio
DOI:
IF: 7.861
应用: WB
反应种属: Human
发表时间: 2021 Aug
-
CDK7 Inhibitor THZ1 Induces the Cell Apoptosis of B-Cell Acute Lymphocytic Leukemia by Perturbing Cellular Metabolism
期刊: Frontiers In Oncology
DOI: 10.3389/fonc.2021.663360
IF: 6.244
应用: WB
反应种属: Human
发表时间: 2021 Apr
-
ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation. Nature communications, 12(1), 2346.
期刊: Nature Communications
DOI:
IF: 12.12
应用: WB
反应种属: Human
发表时间: 2021 Apr
-
Features of Nuclear Export Signals of NS2 Protein of Influenza D Virus
期刊: Viruses
DOI:
IF: 3.816
应用: Co-IP
反应种属: Human
发表时间: 2020 Sep
-
Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation
期刊: Molecular Cell
DOI:
IF: 15.584
应用: WB,IP
反应种属: Mouse
发表时间: 2020 Oct
-
Endoplasmic Reticulum Stress-Mediated p62 Downregulation Inhibits Apoptosis via c-Jun Upregulation
期刊: Biomolecules & Therapeutics
DOI: 10.4062/biomolther.2020.089
IF: 3.47
应用: WB
反应种属: Human
发表时间: 2020 Oct
-
The RCAN1.4-calcineurin/NFAT signaling pathway is essential for hypoxic adaption of intervertebral discs
期刊: Experimental & Molecular Medicine
DOI:
IF: 4.743
应用: WB,IP
反应种属: Human
发表时间: 2020 May
-
Protein Interactions Network of Porcine Circovirus Type 2 Capsid With Host Proteins
期刊: Frontiers In Microbiology
DOI:
IF: 4.235
应用: WB
反应种属: pig
发表时间: 2020 Jun
-
AMPK facilitates intestinal long-chain fatty acid uptake by manipulating CD36 expression and translocation
期刊: FASEB Journal
DOI: 10.1096/fj.201901994R
IF: 4.966
应用: WB
反应种属: Human
发表时间: 2020 Feb
-
Nucleolar protein NPM1 is essential for circovirus replication by binding to viral capsid
期刊: Virulence
DOI:
IF: 5.542
应用: WB,Co-IP
反应种属: Human
发表时间: 2020 Dec
-
Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function
期刊: Frontiers In Cell And Developmental Biology
DOI:
IF: 5.201
应用: IP
反应种属: Human
发表时间: 2020 Dec
-
SCFFBXO28-mediated self-ubiquitination of FBXO28 promotes its degradation
期刊: Cellular Signalling
DOI: 10.1016/j.cellsig.2019.109440
IF: 3.388
应用: WB
反应种属: Human
发表时间: 2019 Oct
-
Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and StressActivated Protein Kinase 1-Dependent Manner
期刊: Infection And Immunity
DOI:
IF: 3.256
应用: WB
反应种属: Human,Mouse
发表时间: 2019 Dec
-
Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress
期刊: Nature Communications
DOI:
IF: 11.878
应用: IP,Co-IP
反应种属: Human
发表时间: 2019 Dec
-
TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction
期刊: Autophagy
DOI:
IF: 11.059
应用: WB
反应种属: Human
发表时间: 2019 Aug
-
The interacting domains in cereblon differentially modulate the immunomodulatory drug-mediated ubiquitination and degradation of its binding partners
期刊: Biochemical And Biophysical Research Communications
DOI: 10.1016/j.bbrc.2018.11.058
IF: 2.559
应用: WB
反应种属: Human
发表时间: 2018 Nov
-
Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages
期刊: Immunity
DOI:
IF: 19.734
应用: WB
反应种属: Species independent
发表时间: 2018 Nov
-
Cereblon suppresses the lipopolysaccharide-induced inflammatory response by promoting the ubiquitination and degradation of c-Jun
期刊: Journal Of Biological Chemistry
DOI: 10.1074/jbc.RA118.002246
IF: 4.011
应用: IF
反应种属: Human
发表时间: 2018 May
-
E4bp4 Regulates Carboxylesterase 2 Enzymes through Repression of the Nuclear Receptor Rev-erbα in Mice
期刊: Biochemical Pharmacology
DOI:
IF: 4.581
应用: WB
反应种属: Mouse
发表时间: 2018 Jun
-
Downregulated NDR1 protein kinase inhibits innate immune response by initiating an miR146a-STAT1 feedback loop
期刊: Nature Communications
DOI:
IF: 12.353
应用: WB
反应种属: Mouse
发表时间: 2018 Jul
-
Dicer interacts with SIRT7 and regulates H3K18 deacetylation in response to DNA damaging agents
期刊: Nucleic Acids Research
DOI:
IF: 9.2
应用: WB
反应种属: Human
发表时间: 2016 May
-
MSX3 switches microglia polarization and protects from inflammation-induced demyelination
期刊: Journal Of Neuroscience
DOI:
IF: 5.3
应用: ChIP
反应种属: Mouse
发表时间: 2015 Apr
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