Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody
Catalog# IRS029
Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody
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mIHC
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Human
概述
产品名称
Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Cytokeratin 7 aa 18-67.
种属反应性
Human
验证应用
mIHC
分子量
Predicted band size: 51 kDa
阳性对照
Human gastric cancer tissue.
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
mIHC
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1:100
靶点
功能
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. Cytokeratin 7 (also known as sarcolectin) agglutinates normal and transformed cells with a high affinity for simple sugars. Cytokeratin 7 also inhibits the synthesis of interferon-dependent secondary proteins thus reversing the antiviral effect of interferon induction and restoring cells to their status ad primum. In normal and transformed cells, Cytokeratin 7 localizes to the membrane.
背景文献
1. Hrudka J. et al. Cytokeratin 7 expression as a predictor of an unfavorable prognosis in colorectal carcinoma. Sci Rep. 2021 Sep
2. Statz E. et al. Cytokeratin 7, GATA3, and SOX-10 is a Comprehensive Panel in Diagnosing Triple Negative Breast Cancer Brain Metastases. Int J Surg Pathol. 2021 Aug
序列相似性
Belongs to the intermediate filament family.
组织特异性
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
翻译后修饰
Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
亚细胞定位
Cytoplasm.
UNIPROT
别名
CK 7 antibody
CK-7 antibody
CK7 antibody
Cytokeratin 7 antibody
Cytokeratin-7 antibody
D15Wsu77e antibody
K2C7 antibody
K2C7_HUMAN antibody
K7 antibody
Keratin 7 antibody
展开图片
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mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Cytokeratin 7 antibody (IRS029) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD21 (IRS019), PD-1, CD14 (IRS015), CD38 (IRS006), aSMA (IRS012) and CK7 (IRS029) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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