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HRP Conjugated Rabbit anti-Human IgG polyclonal Antibody

RMB: 200

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Catalog# HA1024

HRP Conjugated Rabbit anti-Human IgG polyclonal Antibody

Application

  • WB

  • ELISA

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

HRP Conjugated Rabbit anti-Human IgG polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Native protein.

种属反应性

Human

验证应用

WB, ELISA

阳性对照

Human IgG.

偶联

HRP

产品特性

形态

Liquid

浓度

2ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:5000-1:20000

  • ELISA

  • Use at an assay dependent concentration.

发表文章中的应用

WB 查看 5 篇文献如下

发表文章中的种属

Human 查看 4 篇文献如下
Rat 查看 1 篇文献如下

靶点

功能

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

背景文献

暂无

UNIPROT

SwissProt: P01857 Human

SwissProt: P01859 Human

SwissProt: P01860 Human

SwissProt: P01861 Human

别名

FLJ39988 antibody

FLJ40036 antibody

FLJ40253 antibody

FLJ40587 antibody

FLJ40789 antibody

FLJ40834 antibody

G1m marker antibody

G2m marker antibody

G3m marker antibody

G4m marker antibody

展开

图片

  • Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Rabbit anti-Human IgG-HRP (<a href="/products/HA1024" style="font-weight: bold;text-decoration: underline;">HA1024</a>) was used for 1 hour at room temperature at 1:10,000 (Lane 1) and 1:5,000 (Lane 2).<br />Lane 1: Human IgG (30 ng per lane).<br />Lane 2: Mouse IgG (30 ng per lane).<br />Exposure time: 1 minutes

    Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Rabbit anti-Human IgG-HRP (HA1024) was used for 1 hour at room temperature at 1:10,000 (Lane 1) and 1:5,000 (Lane 2).
    Lane 1: Human IgG (30 ng per lane).
    Lane 2: Mouse IgG (30 ng per lane).
    Exposure time: 1 minutes

  • Immobilized human IgG protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (<a href="/products/HA1024" style="font-weight: bold;text-decoration: underline;">HA1024</a>) for 1 hour at 25℃.

    Immobilized human IgG protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA1024) for 1 hour at 25℃.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
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