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HRP Conjugated Rabbit anti-Goat IgG polyclonal Antibody

RMB: 200

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Catalog# HA1005

HRP Conjugated Rabbit anti-Goat IgG polyclonal Antibody

Application

  • WB

  • ELISA

Reactivity

  • Goat

Conjugation

  • HRP

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

HRP Conjugated Rabbit anti-Goat IgG polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Recombinant protein.

种属反应性

Goat

验证应用

WB, ELISA

偶联

HRP

产品特性

形态

Liquid

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.5%BSA, 40% Glycerol.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:5,000

  • ELISA

  • 1:10,000

靶点

功能

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

背景文献

暂无

图片

  • Western blot analysis of Rabbit anti-Goat IgG on Got IgG lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Rabbit anti Goat IgG-HRP Secondary Antibody (<a href="/products/HA1005" style="font-weight: bold;text-decoration: underline;">HA1005</a>) at 1:5,000 (Lane A),1:10,000 (Lane B),1:20,000 (Lane C)dilution was used for 2 hour at room temperature.

    Western blot analysis of Rabbit anti-Goat IgG on Got IgG lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Rabbit anti Goat IgG-HRP Secondary Antibody (HA1005) at 1:5,000 (Lane A),1:10,000 (Lane B),1:20,000 (Lane C)dilution was used for 2 hour at room temperature.

  • The Cross-reactions of the secondary antibody (<a href="/products/HA1005" style="font-weight: bold;text-decoration: underline;">HA1005</a>) with Mouse IgG and Rabbit IgG.<br />Western blot analysis of Rabbit anti-Goat IgG on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Rabbit anti Goat IgG-HRP Secondary Antibody (<a href="/products/HA1005" style="font-weight: bold;text-decoration: underline;">HA1005</a>) at 1:5,000 dilution was used for 2 hour at room temperature.<br />Positive control: <br />Lane A: Mouse IgG<br />Lane B: Rabbit IgG

    The Cross-reactions of the secondary antibody (HA1005) with Mouse IgG and Rabbit IgG.
    Western blot analysis of Rabbit anti-Goat IgG on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Rabbit anti Goat IgG-HRP Secondary Antibody (HA1005) at 1:5,000 dilution was used for 2 hour at room temperature.
    Positive control:
    Lane A: Mouse IgG
    Lane B: Rabbit IgG

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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