DDX4 Recombinant Rabbit Monoclonal Antibody [PSH08-54]
概述
产品名称
DDX4 Recombinant Rabbit Monoclonal Antibody [PSH08-54]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human DDX4 aa 201-724.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IHC-Fr
分子量
Predicted band size: 79 kDa
阳性对照
Mouse testis tissue lysate, Rat testis tissue lysate, human testis tissue, mouse testis tissue, mouse ovary tissue.
偶联
unconjugated
克隆号
PSH08-54
反应性数据
| WB | IHC-P | IHC-Fr | |
|---|---|---|---|
| Human |
|
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| Mouse |
|
|
|
| Rat |
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IHC-P
-
1:1,000-1:5,000
-
IHC-Fr
-
1:500
靶点
功能
The Vasa gene is a member of the DEAD box family of RNA helicases in Drosophila melanogaster. Its human ortholog, Ddx4, is located on human chromosome 5q. It is syntenic to mouse chromosome 13, where the mouse vasa gene is located. The gene is conserved in many invertebrates and vertebrate species such as Caenorhabditis elegans, Xenopus, Zebrafish, flatworms, echinoderms, molluscs, nematodes, mice and rats as an important part of germ line maintenance and function. One of main function of Vasa protein is in germ cell determination and function. It uses ATP dependent RNA helicase catalytic activity to regulate the translation of multiple mRNAs. Vasa unwinds the duplex RNA by binding and bending short stretches of the duplex in a non-processive manner. The conserved domain may act as chaperones by unwinding RNA secondary structures and refolding properly. pre-mRNA splicing, ribosome biogenesis, nuclear export, translational regulation and degradation. Vasa was found to bind RNA in a sequence-specific manner. In the Drosophila embryos, Vasa binds the Uracil rich motif of the mei-P26 UTR. A mutation in Vasa reduced the interaction of between Mei-P26 and initiation factor elF58 which in turn significantly reduced translation of the gene. Recent evidence in invertebrates suggests that Vasa has a role in multipotent stem cells, but the exact function is unknown.
背景文献
1. Miao Y et al. DDX4 enhances antiviral activity of type I interferon by disrupting interaction of USP7/SOCS1 and promoting degradation of SOCS1. mBio. 2024 Mar
2. Xu C et al. Building RNA-protein germ granules: insights from the multifaceted functions of DEAD-box helicase Vasa/Ddx4 in germline development. Cell Mol Life Sci. 2021 Dec
亚细胞定位
Cytoplasm, perinuclear region.
别名
DDX 4 antibody
Ddx4 antibody
DDX4_HUMAN antibody
DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody
DEAD box protein 4 antibody
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody
MVH antibody
Probable ATP dependent RNA helicase DDX4 antibody
Probable ATP-dependent RNA helicase DDX4 antibody
VASA antibody
展开图片
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Immunofluorescence analysis of frozen mouse testis tissue with Rabbit anti-DDX4 antibody (HA723000) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723000, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
☑ Relative expression (RE)
Western blot analysis of DDX4 on different lysates with Rabbit anti-DDX4 antibody (HA723000) at 1/2,000 dilution.
Lane 1: Mouse testis tissue lysate
Lane 2: Mouse spleen tissue lysate (negative)
Lane 3: Mouse brain tissue lysate (negative)
Lane 4: Rat testis tissue lysate
Lane 5: Rat spleen tissue lysate (negative)
Lane 6: Rat brain tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 79 kDa
Observed band size: 75 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723000) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DDX4 antibody (HA723000) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723000) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-DDX4 antibody (HA723000) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723000) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-DDX4 antibody (HA723000) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723000) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Sodium nitrite impairs reproduction in female mice by disrupting ovarian steroid hormone synthesis and lipid metabolic pathways
期刊: Ecotoxicology And Environmental Safety
DOI: 10.1016/j.ecoenv.2025.119631
IF: 6.1
应用: WB
反应种属: Mouse
发表时间: 2026 Jan
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