Iba1 Recombinant Antibody [JM36-62] - Guinea pig IgG2 (Chimeric)

Immunofluorescence analysis of frozen mouse cerebellum tissue labeling Iba1 with Guinea pig anti-Iba1 antibody (HA601368).

Important Notice: Antigen retrieval is not required before IHC-Fr staining.

The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601368, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Rabbit anti-Guinea pig IgG (H&L)-Alexa Fluor® 594 was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Mouse anti-NeuN antibody (HA601111, green) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution.

Immunofluorescence analysis of frozen mouse brain tissue labeling Iba1 with Guinea pig anti-Iba1 antibody (HA601368) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601368, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Rabbit anti-Guinea pig IgG (H&L)-Alexa Fluor® 594 was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Iba1 with Guinea pig anti-Iba1 antibody (HA601368) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601368, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Rabbit anti-Guinea pig IgG (H&L)-Alexa Fluor® 594 was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunofluorescence analysis of frozen rat brain tissue labeling Iba1 with Guinea pig anti-Iba1 antibody (HA601368) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601368, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Rabbit anti-Guinea pig IgG (H&L)-Alexa Fluor® 594 was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Western blot analysis of Iba1 on THP-1 cell lysates with Guinea pig anti-Iba1 antibody (HA601368) at 1/1,000 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 17 kDa
Observed band size: 12 kDa

Exposure time: 2 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601368) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Rabbit anti-Guinea pig IgG - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature.