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Iba1 Recombinant Antibody [JM36-62] - Chicken IgY (Chimeric)

RMB: 666 特惠 1500

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Catalog# HA601376

Iba1 Recombinant Antibody [JM36-62] - Chicken IgY (Chimeric)

Application

  • IHC-Fr

Reactivity

  • Mouse

概述

产品名称

Iba1 Recombinant Antibody [JM36-62] - Chicken IgY (Chimeric)

抗体类型

Recombinant Chimeric Antibody

免疫原

Synthetic peptide within N-terminal human Iba1.

种属反应性

Mouse

验证应用

IHC-Fr

分子量

Predicted band size: 17 kDa

阳性对照

Mouse cerebellum tissue.

偶联

unconjugated

克隆号

JM36-62

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

纯化方式

Protein A affinity purified.

应用稀释度

  • IHC-Fr

  • 1:500

靶点

功能

Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).

背景文献

1. Hennessy E et al. Systemic TNF-a produces acute cognitive dysfunction and exaggerated sickness behavior when superimposed upon progressive neurodegeneration. Brain Behav Immun 59:233-244 (2017).

2. Arentsen T et al. The bacterial peptidoglycan-sensing molecule Pglyrp2 modulates brain development and behavior. Mol Psychiatry 22:257-266 (2017).

亚细胞定位

Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup.

UNIPROT #

SwissProt: P55008 Human

SwissProt: O70200 Mouse

SwissProt: P55009 Rat

别名

AIF 1 antibody

AIF-1 antibody

Aif1 antibody

AIF1 protein antibody

AIF1_HUMAN antibody

Allograft inflammatory factor 1 antibody

Allograft inflammatory factor 1 splice variant G antibody

allograft inflammatory factor-1 splice variant Hara-1 antibody

balloon angioplasty responsive transcription antibody

BART 1 antibody

展开

图片

  • Immunofluorescence analysis of frozen mouse cerebellum tissue with Chicken anti-Iba1 antibody (<a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>) at 1/500 dilution.<br /><br />Important Notice: Antigen retrieval is not required before IHC-Fr staining. <br /><br />The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Chicken IgY H&L-Alexa Fluor&reg; 488 was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).<br /><br />Rabbit anti-MAG antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>, red) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunofluorescence analysis of frozen mouse cerebellum tissue with Chicken anti-Iba1 antibody (HA601376) at 1/500 dilution.

    Important Notice: Antigen retrieval is not required before IHC-Fr staining.

    The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601376, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Chicken IgY H&L-Alexa Fluor® 488 was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Rabbit anti-MAG antibody (HA721818, red) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

  • Immunofluorescence analysis of frozen mouse cerebellum tissue with Chicken anti-Iba1 antibody (<a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>) at 1/500 dilution.<br /><br />Important Notice: Antigen retrieval is not required before IHC-Fr staining. <br /><br />The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Chicken IgY H&L-Alexa Fluor&reg; 488 was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).<br /><br />Rabbit anti-PSD95 antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>, red) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunofluorescence analysis of frozen mouse cerebellum tissue with Chicken anti-Iba1 antibody (HA601376) at 1/500 dilution.

    Important Notice: Antigen retrieval is not required before IHC-Fr staining.

    The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601376, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Chicken IgY H&L-Alexa Fluor® 488 was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Rabbit anti-PSD95 antibody (ET1602-20, red) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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