intestinal alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [PSH17-83]
Catalog# HA723952
intestinal alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [PSH17-83]
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IHC-P
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Human
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Mouse
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Rat
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HA751642
不含抗保成分
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unconjugated
概述
产品名称
intestinal alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [PSH17-83]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human ALPI aa 1-503.
种属反应性
Human, Mouse, Rat
验证应用
IHC-P
分子量
Predicted band size: 57 kDa
阳性对照
Human small intestine tissue, human placenta tissue, mouse small intestine tissue, rat small intestine tissue.
偶联
unconjugated
克隆号
PSH17-83
反应性数据
| IHC-P | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-P
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1:1,000-1:30,000
靶点
功能
Alkaline phosphatase, intestinal, also known as ALPI, is a type of alkaline phosphatase that in humans is encoded by the ALPI gene. Intestinal alkaline phosphatase is an endogenous enzyme that maintains gut homeostasis. It detoxifyies bacterial toxins, dephosphorylates phosphorylated nucleotides, regulates lipid absorption in the intestine, and regulates the microbiome in the intestine. In addition to these functions, intestinal alkaline phosphatase can also modulate bicarbonate secretion and can modulate the pH of the duodenum.
背景文献
1. Santos GM et al. Intestinal Alkaline Phosphatase: A Review of This Enzyme Role in the Intestinal Barrier Function. Microorganisms. 2022 Mar
2. Singh SB et al. Role of Intestinal Alkaline Phosphatase in Innate Immunity. Biomolecules. 2021 Nov
亚细胞定位
Cell membrane.
别名
Alkaline phosphatase intestinal antibody
Alkaline phosphomonoesterase antibody
Alpi antibody
Glycerophosphatase antibody
IAP antibody
Intestinal alkaline phosphatase antibody
Intestinal-type alkaline phosphatase antibody
Kasahara isozyme antibody
OTTHUMP00000164357 antibody
PPBI_HUMAN antibody
图片
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Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-intestinal alkaline phosphatase antibody (HA723952) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723952) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-intestinal alkaline phosphatase antibody (HA723952) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723952) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-intestinal alkaline phosphatase antibody (HA723952) at 1/30,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723952) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-intestinal alkaline phosphatase antibody (HA723952) at 1/30,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723952) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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