alpha Tubulin Rabbit Polyclonal Antibody

功能

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

背景文献

1. "Alternative 5\' exons either provide or deny an initiator methionine codon to the same alpha-tubulin coding region." Dobner P.R., Kislauskis E., Wentworth B.M., Villa-Komaroff L. Nucleic Acids Res. 15:199-218(1987)

2. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008)

序列相似性

Belongs to the tubulin family.

翻译后修饰

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.; Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.

亚细胞定位

Cytoplasm, cytoskeleton, Microtubule.

UNIPROT

SwissProt: P68366 Human

SwissProt: Q5XIF6 Rat

别名

TUBA1 antibody

TUBA1A antibody

TUBA2 antibody

TUBA3 antibody

TUBA3C antibody

TUBA4A antibody

Tubulin, alpha 1a antibody

Tubulin, alpha 3c antibody

Tubulin, alpha 4a antibody