Wnt3a Recombinant Rabbit Monoclonal Antibody [PSH19-96]
Catalog# HA724116
Wnt3a Recombinant Rabbit Monoclonal Antibody [PSH19-96]
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WB
-
IHC-P
-
IP
-
Human
-
Mouse
概述
产品名称
Wnt3a Recombinant Rabbit Monoclonal Antibody [PSH19-96]
抗体类型
Recombinant Rabbit monoclonal Antibody
种属反应性
Human, Mouse
验证应用
WB, IHC-P, IP
分子量
Predicted band size: 39 kDa
阳性对照
HCT 116 cell lysates, mouse E14.5 embryo tissue.
偶联
unconjugated
克隆号
PSH19-96
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
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IHC-P
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1:200
-
IP
-
1-2μg/sample
靶点
功能
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region.
背景文献
1. Kyun ML. et. al. Wnt3a Stimulation Promotes Primary Ciliogenesis through beta-Catenin Phosphorylation-Induced Reorganization of Centriolar Satellites. Cell Rep. 2020 Feb
2. Lv X. et. al. Overexpression of miR-27b-3p Targeting Wnt3a Regulates the Signaling Pathway of Wnt/beta-Catenin and Attenuates Atrial Fibrosis in Rats with Atrial Fibrillation. Oxid Med Cell Longev. 2019 Apr
亚细胞定位
Secreted, extracellular space, extracellular matrix.
别名
Protein Wnt 3a Precursor antibody
Protein Wnt-3a antibody
Wingless type MMTV integration site family member 3A antibody
Wnt 3a antibody
wnt3a antibody
WNT3A protein antibody
WNT3A_HUMAN antibody
图片
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Western blot analysis of Wnt3a on HCT 116 cell lysates with Rabbit anti-Wnt3a antibody (HA724116) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724116) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue with Rabbit anti-Wnt3a antibody (HA724116) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724116) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Wnt3a was immunoprecipitated from 0.2 mg HCT 116 cell lysate with HA724116 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724116 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HCT 116 cell lysate (input)
Lane 2: HA724116 IP in HCT 116 cell lysate
Lane 3: Rabbit IgG instead of HA724116 in HCT 116 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 3 minutes; ECL: K1802
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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