Total OXPHOS Antibody Cocktail Recombinant Rabbit Multiclonal Antibody [PSH15-50]
Catalog# HA723756
Total OXPHOS Antibody Cocktail Recombinant Rabbit Multiclonal Antibody [PSH15-50]
-
WB
-
IHC-P
-
Human
-
unconjugated
概述
产品名称
Total OXPHOS Antibody Cocktail Recombinant Rabbit Multiclonal Antibody [PSH15-50]
抗体类型
Recombinant Rabbit multiclonal
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 18/22/29/48/54 kDa
阳性对照
HEK-293 cell lysate, HeLa cell lysate, A549 cell lysate, K-562 cell lysate, MCF7 cell lysate, human breast carcinoma tissue, human liver tissue.
偶联
unconjugated
克隆号
PSH15-50
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IHC-P
-
1:200
靶点
背景文献
暂无
序列相似性
Belongs to the ATPase alpha/beta chains family.
组织特异性
Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
翻译后修饰
The N-terminus is blocked.; Acetylated on lysine residues. BLOC1S1 is required for acetylation.
亚细胞定位
Mitochondrion inner membrane.
UNIPROT
别名
ASHI antibody
ATP synthase subunit alpha antibody
Atp5a1 antibody
ATP5AL2 antibody
COII antibody
Complex I ASHI subunit antibody
COX 2 antibody
COX II antibody
COX2 antibody
COXII antibody
展开图片
-
Western blot analysis of Total OXPHOS Antibody Cocktail on different lysates with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (HA723756) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: A549 cell lysate
Lane 4: K-562 cell lysate
Lane 5: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18/22/29/48/54 kDa
Observed band size: 18/22/29/48/54 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723756) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (HA723756) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723756) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (HA723756) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723756) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
浙公网安备 33019202000643号