TIMP1 Recombinant Rabbit Monoclonal Antibody [PSH21-64]
Catalog# HA724231
TIMP1 Recombinant Rabbit Monoclonal Antibody [PSH21-64]
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WB
-
IHC-P
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Human
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Monkey
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HA751803
不含抗保成分
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unconjugated
概述
产品名称
TIMP1 Recombinant Rabbit Monoclonal Antibody [PSH21-64]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human TIMP1 aa 1-207.
种属反应性
Human, Monkey
验证应用
WB, IHC-P
分子量
Predicted band size: 23 kDa
阳性对照
HT-29 cell lysate, SKOV-3 cell lysate, COS-1 cell lysate, human medullary thyroid carcinoma tissue, human lung cancer tissue.
偶联
unconjugated
克隆号
PSH21-64
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:5,000
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IHC-P
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1:200-1:1,000
靶点
功能
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
背景文献
1. Guccini I et al. Senescence Reprogramming by TIMP1 Deficiency Promotes Prostate Cancer Metastasis. Cancer Cell. 2021 Jan
2. Schoeps B et al. TIMP1 Triggers Neutrophil Extracellular Trap Formation in Pancreatic Cancer. Cancer Res. 2021 Jul
亚细胞定位
Secreted.
UNIPROT
别名
Clgi antibody
Collagenase inhibitor antibody
Collagenase inhibitor, Human antibody
EPA antibody
EPO antibody
Erythroid Potentiating Activity antibody
Erythroid-potentiating activity antibody
Fibroblast collagenase inhibitor antibody
FLJ90373 antibody
HCI antibody
展开图片
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Western blot analysis of TIMP1 on different lysates with Rabbit anti-TIMP1 antibody (HA724231) at 1/5,000 dilution.
Lane 1: HT-29 cell lysate
Lane 2: SKOV-3 cell lysate
Lane 3: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 30 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724231) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human medullary thyroid carcinoma tissue with Rabbit anti-TIMP1 antibody (HA724231) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724231) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-TIMP1 antibody (HA724231) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724231) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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