TGF beta Receptor II Rabbit Polyclonal Antibody
Catalog# ER1917-66
TGF beta Receptor II Rabbit Polyclonal Antibody
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
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Chicken
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Pig
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Cow
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Horse
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Rabbit
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Sheep
概述
产品名称
TGF beta Receptor II Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human TGF beta Receptor II: 241-330/567
种属反应性
Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Sheep
验证应用
IHC-P, IF-Tissue
分子量
62 kDa
阳性对照
Kidney (Mouse) Lysate, Brain(Mouse) Lysate, Brain(Rat) Lysate, HepG2 Cell Lysate.
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
存储缓冲液
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-P
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1:100-500
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IF-Tissue
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1:100-500
发表文章中的应用
发表文章中的种属
| Human | See 1 publications below |
| Mouse | See 1 publications below |
靶点
功能
Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways.
背景文献
暂无
亚细胞定位
Cell membrane; Single-pass type I membrane protein.
UNIPROT #
别名
AAT3 antibody
FAA3 antibody
LDS1B antibody
LDS2 antibody
LDS2B antibody
MFS2 antibody
RIIC antibody
TAAD2 antibody
TbetaR II antibody
TbetaR-II antibody
展开图片
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Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (ER1917-66) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (ER1917-66) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (ER1917-66) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (ER1917-66) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (ER1917-66) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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The Upregulation of Leucine-Rich Repeat Containing 1 Expression Activates Hepatic Stellate Cells and Promotes Liver Fibrosis by Stabilizing Phosphorylated Smad2/3
Author: Wang Yake,et al
PMID: 38473980
应用: WB
反应种属: Mouse
发表时间: 2024 Mar
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Citation
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circGRHPR inhibits aberrant epithelial-mesenchymal transformation progression of lung epithelial cells associated with idiopathic pulmonary fibrosis
Author: Wu Wensi,et al
PMID: 38267743
应用: WB
反应种属: Human
发表时间: 2024 Jan
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Citation
同靶点&同通路的产品
TGF beta Receptor II Recombinant Rabbit Monoclonal Antibody [JE59-90]
Application: WB
Reactivity: Human,Rat
Conjugate: unconjugated
