TGF beta Receptor I Recombinant Rabbit Monoclonal Antibody [PSH14-14]
Catalog# HA723604
TGF beta Receptor I Recombinant Rabbit Monoclonal Antibody [PSH14-14]
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WB
-
IP
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Human
-
Mouse
-
Rat
概述
产品名称
TGF beta Receptor I Recombinant Rabbit Monoclonal Antibody [PSH14-14]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human TGF beta Receptor I aa 34-503.
种属反应性
Human, Mouse, Rat
验证应用
WB, IP
分子量
Predicted band size: 56 kDa
阳性对照
THP-1 cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, RAW264.7 cell lysate, C6 cell lysate.
偶联
unconjugated
克隆号
PSH14-14
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
-
1:2,000
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IP
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1-2μg/sample
靶点
功能
Transforming growth factor beta receptor I (activin A receptor type II-like kinase, 53kDa) is a membrane-bound TGF beta receptor protein of the TGF-beta receptor family for the TGF beta superfamily of signaling ligands. TGFBR1 is its human gene. The protein encoded by this gene forms a heteromeric complex with type II TGF-β receptors when bound to TGF-β, transducing the TGF-β signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys–Dietz aortic aneurysm syndrome (LDS, LDAS).
背景文献
1. Wang J et al. Nestin promotes pulmonary fibrosis via facilitating recycling of TGF-beta receptor I. Eur Respir J. 2022 May
2. Ghorbaninejad M et al. TGF-beta receptor I inhibitor may restrict the induction of EMT in inflamed intestinal epithelial cells. Exp Biol Med (Maywood). 2023 Apr
亚细胞定位
Cell membrane, Cell junction, tight junction, Cell surface, Membrane raft.
别名
AAT 5 antibody
AAT5 antibody
Activin A receptor type II like kinase 53kDa antibody
Activin A receptor type II like kinase antibody
Activin A receptor type II like kinase, 53kD antibody
Activin A receptor type II like protein kinase of 53kD antibody
activin A receptor type II-like kinase, 53kDa antibody
activin A receptor type II-like protein kinase of 53kD antibody
Activin receptor like kinase 5 antibody
Activin receptor-like kinase 5 antibody
展开图片
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Western blot analysis of TGF beta Receptor I on different lysates with Rabbit anti-TGF beta Receptor I antibody (HA723604) at 1/2,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: Neuro-2a cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 50/37 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723604) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
TGF beta Receptor I was immunoprecipitated from 0.2 mg THP-1 cell lysate with HA723604 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723604 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: THP-1 cell lysate (input)
Lane 2: HA723604 IP in THP-1 cell lysate
Lane 3: Rabbit IgG instead of HA723604 in THP-1 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 7 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
TGF beta Receptor I Recombinant Rabbit Monoclonal Antibody [PSH14-14] - BSA and Azide free
Application: WB,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
