STAT1 Recombinant Mouse Monoclonal Antibody [G3-B11-R]

Western blot analysis of STAT1 on different lysates with Mouse anti-STAT1 antibody (HA601309) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: A431 cell lysate
Lane 3: HeLa cell lysate
Lane 4: A549 cell lysate
Lane 5: SK-Br-3 cell lysate
Lane 6: SK-MEL-28 cell lysate
Lane 7: MCF7 cell lysate
Lane 8: HT-29 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 87/83 kDa
Observed band size: 87/83 kDa

Exposure time: 30 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601309) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-STAT1 antibody (HA601309) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601309) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-STAT1 antibody (HA601309) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601309) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.