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SQSTM1 / p62 Rabbit Polyclonal Antibody

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Catalog# R1309-8

SQSTM1 / p62 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

SQSTM1 / p62 Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within human TESPA1 aa 27-91.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Cell, IHC-P

分子量

62kDa

阳性对照

Hela, F9, HepG2, human ovary carcinoma tissue

偶联

unconjugated

RRID

AB_3073248

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:200

  • IHC-P

  • 1:200

靶点

功能

Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress and autophagy. It was first identified as a protein that binds to the SH2 domain of p56Lck and independently found to interact with PKCζ. SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome. Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway. It may play a role in titin/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. P62 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.

背景文献

1. "p62 overexpression in breast tumors and regulation by prostate-derived Ets factor in breast cancer cells." Thompson H.G.R., Harris J.W., Wold B.J., Lin F., Brody J.P. Oncogene 22:2322-2333(2003)

2. "Ubiquitin recognition by the ubiquitin-associated domain of p62 involves a novel conformational switch." Long J., Gallagher T.R., Cavey J.R., Sheppard P.W., Ralston S.H., Layfield R., Searle M.S. J. Biol. Chem. 283:5427-5440(2008)

组织特异性

Ubiquitously expressed.

翻译后修饰

Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN. Phosphorylation at Ser-403 by ULK1 is stimulated by SESN2. Phosphorylated at Ser-403 by TBK1, leading to promote relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Phosphorylation at Ser-349 by MTOR promotes interaction with KEAP1 and inactivation of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes (By similarity).; Ubiquitinated by RNF26: ubiquitinated SQSTM1 attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Deubiquitination by USP15 releases target vesicles for fast transport into the cell periphery. Ubiquitinated by the BCR(KEAP1) complex at Lys-420, increasing SQSTM1 sequestering activity and promoting its degradation. Ubiquitinated via 'Lys-29' and 'Lys-33'-linked polyubiquitination leading to xenophagic targeting of bacteria and inhibition of their replication.

亚细胞定位

Cytoplasm

UNIPROT

SwissProt: Q13501 Human

别名

A170 antibody

DMRV antibody

EBI 3 associated protein of 60 kDa antibody

EBI 3 associated protein p60 antibody

EBI3 associated protein of 60 kDa antibody

EBI3 associated protein p60 antibody

EBI3-associated protein of 60 kDa antibody

EBIAP antibody

FTDALS3 antibody

MGC127197 antibody

展开

图片

  • Western blot analysis on Hela (1) and F9 (2) cell lysates using anti-SQSTM1 rabbit polyclonal antibodies.

    Western blot analysis on Hela (1) and F9 (2) cell lysates using anti-SQSTM1 rabbit polyclonal antibodies.

  • ICC staining SQSTM1 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

    ICC staining SQSTM1 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

  • Immunohistochemical analysis of paraffin-embedded human ovary carcinoma tissue using anti- SQSTM1 rabbit polyclonal antibody.

    Immunohistochemical analysis of paraffin-embedded human ovary carcinoma tissue using anti- SQSTM1 rabbit polyclonal antibody.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Alternative Products

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SQSTM1 / p62 Recombinant Rabbit Monoclonal Antibody [PS00-61]

Application: WB,IHC-P,IF-Cell,FC,IF-Tissue,IHC-Fr

Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig

Conjugate: unconjugated

同靶点 & 同通路的产品

metafield image

SQSTM1 / p62 Mouse Monoclonal Antibody [10-E10]

Application: IF-Cell,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

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