Phospho-Rb (S780) Recombinant Rabbit Monoclonal Antibody [JE44-61]
Catalog# HA721513
Phospho-Rb (S780) Recombinant Rabbit Monoclonal Antibody [JE44-61]
-
WB
-
Human
-
Mouse
-
Rat
-
unconjugated
概述
产品名称
Phospho-Rb (S780) Recombinant Rabbit Monoclonal Antibody [JE44-61]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phosphopeptide corresponding to resudues surrounding Ser780 of human Rb protein.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 106 kDa
阳性对照
Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours whole cell lysate.
偶联
unconjugated
克隆号
JE44-61
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
靶点
功能
The retinoblastoma protein (protein name abbreviated Rb; gene name abbreviated Rb, RB or RB1) is a tumor suppressor protein that is dysfunctional in several major cancers. One function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. When the cell is ready to divide, pRb is phosphorylated, inactivating it, and the cell cycle is allowed to progress. It is also a recruiter of several chromatin remodeling enzymes such as methylases and acetylases. pRb belongs to the pocket protein family, whose members have a pocket for the functional binding of other proteins. Should an oncogenic protein, such as those produced by cells infected by high-risk types of human papillomavirus, bind and inactivate pRb, this can lead to cancer. The RB gene may have been responsible for the evolution of multicellularity in several lineages of life including animals.
背景文献
1. Linn P et al. Targeting RB1 Loss in Cancers. Cancers (Basel). 2021 Jul
2. Yao Y et al. Novel insights into RB1 mutation. Cancer Lett. 2022 Oct
亚细胞定位
Nucleus.
别名
Exon 17 tumor GOS561 substitution mutation causes premature stop antibody
GOS563 exon 17 substitution mutation causes premature stop antibody
OSRC antibody
Osteosarcoma antibody
p105-Rb antibody
P105RB antibody
PP105 antibody
pp110 antibody
PPP1R130 antibody
pRb antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of Phospho-Rb (S780) on different lysates with Rabbit anti-Phospho-Rb (S780) antibody (HA721513) at 1/1,000 dilution.
Lane 1: Jurkat serum starved for 44 hours whole cell lysate
Lane 2: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours whole cell lysate
Lane 3: Jurkat serum starved for 44 hours then treated with λpp for 1 hour whole cell lysate
Lane 4: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours then treated with λpp for 1 hour whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 1 minute 59 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721513) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Phospho-Rb (S780) on NIH/3T3 cell lysates with Rabbit anti-Phospho-Rb (S780) antibody (HA721513) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721513) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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