Phospho-MEK1 (T292) Recombinant Rabbit Monoclonal Antibody [SD2088]
Catalog# ET1612-42
Phospho-MEK1 (T292) Recombinant Rabbit Monoclonal Antibody [SD2088]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
Phospho-MEK1 (T292) Recombinant Rabbit Monoclonal Antibody [SD2088]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Thr292 of Human MEK1 aa 271-320 / 393.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 43 kDa
阳性对照
HeLa cell lysate, HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate, PC-12 cell lysate, PC-12 treated with 100ng/mL Nocodazole for 18 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 100ng/mL Nocodazole for 18 hours cell lysate, mouse brain tissue.
偶联
unconjugated
克隆号
SD2088
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IHC-P
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1:200
靶点
功能
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK 5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.
背景文献
1. Chou YY et al. Colocalization of different influenza viral RNA segments in the cytoplasm before viral budding as shown by single-molecule sensitivity FISH analysis. PLoS Pathog 9:e1003358 (2013).
2. Nath S et al. MUC1 induces drug resistance in pancreatic cancer cells via upregulation of multidrug resistance genes. Oncogenesis 2:e51 (2013).
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
组织特异性
Widely expressed, with extremely low levels in brain.
翻译后修饰
Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (BRAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK. Autophosphorylated at Ser-218 and Ser-222, autophosphosphorylation is promoted by NEK10 following UV irradiation.; Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
亚细胞定位
Cytoplasm, Nucleus, Membrane.
别名
Dual specificity mitogen activated protein kinase kinase 1 antibody
Dual specificity mitogen-activated protein kinase kinase 1 antibody
ERK activator kinase 1 antibody
MAP kinase kinase 1 antibody
MAP kinase/Erk kinase 1 antibody
MAP2K1 antibody
MAPK/ERK kinase 1 antibody
MAPKK 1 antibody
MAPKK1 antibody
MEK 1 antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of Phospho-MEK1 (T292) on different lysates with Rabbit anti-Phospho-MEK1 (T292) antibody (ET1612-42) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate
Lane 3: PC-12 cell lysate
Lane 4: PC-12 treated with 100ng/mL Nocodazole for 18 hours cell lysate
Lane 5: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate, then the membrane treated with λpp for 1 hour
Lane 6: PC-12 treated with 100ng/mL Nocodazole for 18 hours cell lysate, then the membrane treated with λpp for 1 hour
Lane 7: NIH/3T3 cell lysate
Lane 8: NIH/3T3 treated with 100ng/mL Nocodazole for 18 hours cell lysate
Lane 9: NIH/3T3 treated with 100ng/mL Nocodazole for 18 hours cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-MEK1 (T292) antibody (ET1612-42) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-42) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
Phospho-MEK1 (S298) Recombinant Rabbit Monoclonal Antibody [SD206-7]
Application: WB,IF-Cell,IF-Tissue,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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