Phospho-AMPK alpha 1 (T183) +AMPK alpha 2 (T172) Recombinant Rabbit Monoclonal Antibody [PSH14-13]

☑ Cell treatment (CT)

Western blot analysis of Phospho-AMPK alpha 1 (T183) +AMPK alpha 2 (T172) on different lysates with Rabbit anti-Phospho-AMPK alpha 1 (T183) +AMPK alpha 2 (T172) antibody (HA723603) at 1/2,000 dilution.

Lane 1: NCI-H1299 cell lysate
Lane 2: NCI-H1299 treated with 0.5μM Oligomycin for 15 minutes cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: SH-SY5Y treated with 0.5μM Oligomycin for 30 minutes cell lysate
Lane 5: C2C12 cell lysate
Lane 6: C2C12 treated with 1mM AICAR for 30 minutes cell lysate
Lane 7: C6 cell lysate
Lane 8: C6 treated with 1μM Oligomycin for 30 minutes cell lysate
Lane 9: NCI-H1299 treated with 0.5μM Oligomycin for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour

Lysates/proteins at 20 µg/Lane.

Predicted band size: 64 kDa
Observed band size: 64 kDa

Exposure time: 59 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723603) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

☑ Cell treatment (CT)

Immunohistochemical analysis of paraffin-embedded human bain tissue untreated / treated with λpp with Rabbit anti-Phospho-AMPK alpha 1 (T183) +AMPK alpha 2 (T172) antibody (HA723603) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723603) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

☑ Cell treatment (CT)

Immunohistochemical analysis of paraffin-embedded mouse bain tissue untreated / treated with λpp with Rabbit anti-Phospho-AMPK alpha 1 (T183) +AMPK alpha 2 (T172) antibody (HA723603) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723603) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

☑ Cell treatment (CT)

Immunohistochemical analysis of paraffin-embedded rat bain tissue untreated / treated with λpp with Rabbit anti-Phospho-AMPK alpha 1 (T183) +AMPK alpha 2 (T172) antibody (HA723603) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723603) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.