PI 3 Kinase p85 alpha Mouse Monoclonal Antibody [A3-D0]
Catalog# M1510-2
PI 3 Kinase p85 alpha Mouse Monoclonal Antibody [A3-D0]
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WB
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IF-Cell
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Human
概述
产品名称
PI 3 Kinase p85 alpha Mouse Monoclonal Antibody [A3-D0]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human PI3-kinase p85 subunit alpha aa 19-219 / 724.
种属反应性
Human
验证应用
WB, IF-Cell
分子量
Predicted band size: 84 kDa
阳性对照
SW480 cell lysate, A431 cell lysate, 293T cell lysate, Jurkat cell lysate, A549 cell lysate.
偶联
unconjugated
克隆号
A3-D0
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
靶点
功能
Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of (p85) and (p110) subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110δ expression is restricted to white blood cells. It has been shown to bind p85α and γ, but it apparently does not phosphorylate these subunits. p110δ seems to have the capacity to autophosphorylate. p110γ does not interact with the p85 subunits. It has been shown to be activated by α and βγ heterotrimeric G proteins.
背景文献
1. Hu J et al. Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling. PLoS One 9:e89352 (2014).
2. Schmidt JW et al. Stat5 regulates the phosphatidylinositol 3-kinase/Akt1 pathway during mammary gland development and tumorigenesis. Mol Cell Biol 34:1363-77 (2014).
序列相似性
Belongs to the PI3K p85 subunit family.
组织特异性
Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
翻译后修饰
Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.; Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear (By similarity). Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.
亚细胞定位
Cytosol, Membrane, Nucleus.
UNIPROT
别名
GRB1 antibody
p85 alpha antibody
p85 antibody
P85A_HUMAN antibody
Phosphatidylinositol 3 kinase associated p 85 alpha antibody
Phosphatidylinositol 3 kinase regulatory 1 antibody
Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
展开图片
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Western blot analysis of PI 3 Kinase p85 alpha on different lysates with Mouse anti-PI 3 Kinase p85 alpha antibody (M1510-2) at 1/1,000 dilution.
Lane 1: SW480 cell lysate
Lane 2: A431 cell lysate
Lane 3: 293T cell lysate
Lane 4: Jurkat cell lysate
Lane 5: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 1 minute 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-2) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of PI 3 Kinase p85 alpha on different lysates with Mouse anti-PI 3 Kinase p85 alpha antibody (M1510-2) at 1/2,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si PI 3 Kinase p85 alpha cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 2 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-2) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of U-937 cells labeling PI 3 Kinase p85 alpha with Mouse anti-PI 3 Kinase p85 alpha antibody (M1510-2) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PI 3 Kinase p85 alpha antibody (M1510-2) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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