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NMDAR2B Rabbit Polyclonal Antibody

RMB: 1500

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Catalog# ER1913-90

NMDAR2B Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

NMDAR2B Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

KLH conjugated synthetic peptide derived from human NMDAR2B 451-550/1484

种属反应性

Human, Rat

验证应用

WB, IHC-P, IF-Tissue

分子量

163 KDa

阳性对照

Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.

偶联

unconjugated

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

存储缓冲液

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500-2000

  • IHC-P

  • 1:100-500

  • IF-tissue

  • 1:100-500

发表文章中的应用

IF 查看 1 篇文献如下
WB 查看 1 篇文献如下

发表文章中的种属

Rat 查看 1 篇文献如下

靶点

功能

NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.

背景文献

暂无

亚细胞定位

Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.

UNIPROT

SwissProt: Q13224 Human

别名

AW490526 antibody

EIEE27 antibody

Glutamate [NMDA] receptor subunit epsilon 2 antibody

Glutamate [NMDA] receptor subunit epsilon-2 antibody

Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody

Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody

Glutamate receptor ionotropic NMDA2B antibody

Glutamate receptor subunit epsilon 2 antibody

Glutamate receptor

ionotropic

展开

图片

  • Sample: A549 Cell Lysate at 40 ug<br />Primary: Anti- NMDAR2B (<a href="/products/ER1913-90" style="font-weight: bold;text-decoration: underline;">ER1913-90</a>) at 1/300 dilution<br />Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution<br />Predicted band size: 163 kD<br />Observed band size: 210 kD

    Sample: A549 Cell Lysate at 40 ug
    Primary: Anti- NMDAR2B (ER1913-90) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 163 kD
    Observed band size: 210 kD

  • Tissue/cell: rat spinal cord; 4% Paraformaldehyde-fixed and paraffin-embedded; <br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; <br />Incubation: Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated(<a href="/products/ER1913-90" style="font-weight: bold;text-decoration: underline;">ER1913-90</a>) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Tissue/cell: rat spinal cord; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated(ER1913-90) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

  • Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; <br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; <br />Incubation: Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated(<a href="/products/ER1913-90" style="font-weight: bold;text-decoration: underline;">ER1913-90</a>) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated(ER1913-90) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

  • Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; <br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; <br />Incubation: Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated(<a href="/products/ER1913-90" style="font-weight: bold;text-decoration: underline;">ER1913-90</a>) 1:200, overnight at 4℃; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37℃.

    Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated(ER1913-90) 1:200, overnight at 4℃; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37℃.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. IF
  2. WB

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