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NAK / TBK1 Mouse Monoclonal Antibody [A8D10]

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Catalog# HA601044

NAK / TBK1 Mouse Monoclonal Antibody [A8D10]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

NAK / TBK1 Mouse Monoclonal Antibody [A8D10]

抗体类型

Mouse Monoclonal Antibody

免疫原

Recombinant protein within human TBK1 aa 530-729/729.

种属反应性

Human, Mouse, Rat

验证应用

WB

分子量

Predicted band size: 84 kDa

阳性对照

HCT 116 cell lysate, K-562 cell lysate, HepG2 cell lysate, MCF7 cell lysate, A-172 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, Mouse lung tissue lysate, Rat lung tissue lysate.

偶联

unconjugated

克隆号

A8D10

RRID

AB_3071768

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG1

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:5,000

发表文章中的应用

WB 查看 1 篇文献如下

发表文章中的种属

Rat 查看 1 篇文献如下
Mouse 查看 1 篇文献如下

靶点

功能

TBK1 (TANK-binding kinase 1) is an enzyme with kinase activity. Specifically, it is a serine / threonine protein kinase. It is encoded by the TBK1 gene in humans. This kinase is mainly known for its role in innate immunity antiviral response. However, TBK1 also regulates cell proliferation, apoptosis, autophagy, and anti-tumor immunity. Insufficient regulation of TBK1 activity leads to autoimmune, neurodegenerative diseases or tumorigenesis. TBK1 is involved in many signaling pathways and forms a node between them. For this reason, regulation of its involvement in individual signaling pathways is necessary. This is provided by adaptor proteins that interact with the dimerization domain of TBK1 to determine its location and access to substrates. Binding to TANK leads to localization to the perinuclear region and phosphorylation of substrates which is required for subsequent production of type I interferons (IFN-I). In contrast, binding to NAP1 and SINTBAD leads to localization in the cytoplasm and involvement in autophagy. Another adaptor protein that determines the location of TBK1 is TAPE. TAPE targets TBK1 to endolysosomes.

背景文献

1. Hu L. et. al. TBK1 Is a Synthetic Lethal Target in Cancer with VHL Loss. Cancer Discov. 2020 Mar

2. Aravamudhan A. et. al. TBK1 regulates YAP/TAZ and fibrogenic fibroblast activation. Am J Physiol Lung Cell Mol Physiol. 2020 May

亚细胞定位

Cytoplasm.

UNIPROT

SwissProt: Q9UHD2 Human

SwissProt: Q9WUN2 Mouse

SwissProt: D4A7D3 Rat

别名

EC 2.7.11.1 antibody

FLJ11330 antibody

FTDALS4 antibody

NAK antibody

NF kappa B activating kinase antibody

NF kB activating kinase antibody

NF-kappa-B-activating kinase antibody

Serine/threonine protein kinase TBK 1 antibody

Serine/threonine protein kinase TBK1 antibody

Serine/threonine-protein kinase TBK1 antibody

展开

图片

  • Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (<a href="/products/HA601044" style="font-weight: bold;text-decoration: underline;">HA601044</a>) at 1/1,000 dilution.<br /><br />Lane 1: HCT 116 cell lysate (20 µg/Lane)<br />Lane 2: K-562 cell lysate (20 µg/Lane)<br />Lane 3: HepG2 cell lysate (20 µg/Lane)<br />Lane 4: MCF7 cell lysate (20 µg/Lane)<br />Lane 5: A-172 cell lysate (20 µg/Lane)<br />Lane 6: HeLa cell lysate (20 µg/Lane)<br />Lane 7: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 8: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 9: Rat testis tissue lysate (40 µg/Lane)<br />Lane 10: Mouse lung tissue lysate (40 µg/Lane)<br />Lane 11: Rat lung tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 84 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601044" style="font-weight: bold;text-decoration: underline;">HA601044</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601044) at 1/1,000 dilution.

    Lane 1: HCT 116 cell lysate (20 µg/Lane)
    Lane 2: K-562 cell lysate (20 µg/Lane)
    Lane 3: HepG2 cell lysate (20 µg/Lane)
    Lane 4: MCF7 cell lysate (20 µg/Lane)
    Lane 5: A-172 cell lysate (20 µg/Lane)
    Lane 6: HeLa cell lysate (20 µg/Lane)
    Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 8: Mouse testis tissue lysate (40 µg/Lane)
    Lane 9: Rat testis tissue lysate (40 µg/Lane)
    Lane 10: Mouse lung tissue lysate (40 µg/Lane)
    Lane 11: Rat lung tissue lysate (40 µg/Lane)

    Predicted band size: 84 kDa
    Observed band size: 80 kDa

    Exposure time: 1 minute; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601044) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (<a href="/products/HA601044" style="font-weight: bold;text-decoration: underline;">HA601044</a>) at 1/500 dilution.<br /><br />Lane 1: A549-si NT cell lysate (20 µg/Lane)<br />Lane 2: A549-si NAK / TBK1 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 84 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601044" style="font-weight: bold;text-decoration: underline;">HA601044</a>) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601044) at 1/500 dilution.

    Lane 1: A549-si NT cell lysate (20 µg/Lane)
    Lane 2: A549-si NAK / TBK1 cell lysate (20 µg/Lane)

    Predicted band size: 84 kDa
    Observed band size: 80 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601044) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB

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