NAK / TBK1 Mouse Monoclonal Antibody [A8D10]
Catalog# HA601044
NAK / TBK1 Mouse Monoclonal Antibody [A8D10]
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WB
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Human
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Mouse
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Rat
概述
产品名称
NAK / TBK1 Mouse Monoclonal Antibody [A8D10]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within human TBK1 aa 530-729/729.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 84 kDa
阳性对照
K562 cell lysate, NIH/3T3 cell lysate, Hela cell lysate, MCF-7 cell lysate, HepG2 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat stomach tissue lysate.
偶联
unconjugated
克隆号
A8D10
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500
靶点
功能
TBK1 (TANK-binding kinase 1) is an enzyme with kinase activity. Specifically, it is a serine / threonine protein kinase. It is encoded by the TBK1 gene in humans. This kinase is mainly known for its role in innate immunity antiviral response. However, TBK1 also regulates cell proliferation, apoptosis, autophagy, and anti-tumor immunity. Insufficient regulation of TBK1 activity leads to autoimmune, neurodegenerative diseases or tumorigenesis. TBK1 is involved in many signaling pathways and forms a node between them. For this reason, regulation of its involvement in individual signaling pathways is necessary. This is provided by adaptor proteins that interact with the dimerization domain of TBK1 to determine its location and access to substrates. Binding to TANK leads to localization to the perinuclear region and phosphorylation of substrates which is required for subsequent production of type I interferons (IFN-I). In contrast, binding to NAP1 and SINTBAD leads to localization in the cytoplasm and involvement in autophagy. Another adaptor protein that determines the location of TBK1 is TAPE. TAPE targets TBK1 to endolysosomes.
背景文献
1. Hu L. et. al. TBK1 Is a Synthetic Lethal Target in Cancer with VHL Loss. Cancer Discov. 2020 Mar
2. Aravamudhan A. et. al. TBK1 regulates YAP/TAZ and fibrogenic fibroblast activation. Am J Physiol Lung Cell Mol Physiol. 2020 May
亚细胞定位
Cytoplasm.
别名
EC 2.7.11.1 antibody
FLJ11330 antibody
FTDALS4 antibody
NAK antibody
NF kappa B activating kinase antibody
NF kB activating kinase antibody
NF-kappa-B-activating kinase antibody
Serine/threonine protein kinase TBK 1 antibody
Serine/threonine protein kinase TBK1 antibody
Serine/threonine-protein kinase TBK1 antibody
展开图片
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Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601044) at 1/500 dilution.
Lane 1: K562 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: Hela cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: HepG2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 80 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601044) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601044) at 1/500 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Rat stomach tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 80 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601044) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601044) at 1/500 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si NAK / TBK1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 80 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601044) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
NAK / TBK1 Mouse Monoclonal Antibody [A8D9]
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
