MUSK Recombinant Rabbit Multiclonal Antibody [PSH03-51] - BSA and Azide free
Catalog# HA750885
MUSK Recombinant Rabbit Multiclonal Antibody [PSH03-51] - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
MUSK Recombinant Rabbit Multiclonal Antibody [PSH03-51] - BSA and Azide free
抗体类型
Recombinant Rabbit multiclonal
免疫原
Recombinant protein within human MUSK aa 1-516 / 869.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 97 kDa
阳性对照
Human brain tissue lysate, mouse brain tissue lysate, mouse skeletal muscle tissue lysate, mouse hippocampus tissue lysate, rat brain tissue lysate, rat skeletal muscle tissue lysate, rat hippocampus tissue lysate, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.
偶联
unconjugated
克隆号
PSH03-51
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:2,000
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IHC-P
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1:500-1:2,000
靶点
功能
Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation.
背景文献
1. Rodolico C et al. MuSK-Associated Myasthenia Gravis: Clinical Features and Management. Front Neurol. 2020 Jul
2. Fish LA et al. Multiple MuSK signaling pathways and the aging neuromuscular junction. Neurosci Lett. 2020 Jul
亚细胞定位
Postsynaptic cell membrane.
别名
CMS9 antibody
FADS antibody
MDK 4 antibody
MDK4 antibody
MGC126323 antibody
MGC126324 antibody
Muscle antibody
Muscle associated receptor tyrosine kinase antibody
Muscle skeletal receptor tyrosine kinase antibody
Muscle skeletal receptor tyrosine protein kinase antibody
展开图片
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Western blot analysis of MUSK on different lysates with Rabbit anti-MUSK antibody (HA750885) at 1/2,000 dilution.
Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Mouse skeletal muscle tissue lysate
Lane 4: Mouse hippocampus tissue lysate
Lane 5: Rat brain tissue lysate
Lane 6: Rat skeletal muscle tissue lysate
Lane 7: Rat hippocampus tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 100 kDa
Exposure time: 50 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750885) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-MUSK antibody (HA750885) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750885) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-MUSK antibody (HA750885) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750885) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-MUSK antibody (HA750885) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750885) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"