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MMP-2 Recombinant Rabbit Monoclonal Antibody [SI15-04]

RMB: 900 特惠 1500

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Catalog# ET1606-4

MMP-2 Recombinant Rabbit Monoclonal Antibody [SI15-04]

Application

  • WB

  • IHC-P

  • IP

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

MMP-2 Recombinant Rabbit Monoclonal Antibody [SI15-04]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human MMP2 aa 101-150 / 660.

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P, IP, IF-Tissue

分子量

Predicted band size: 74 kDa

阳性对照

U-87 MG cell lysate, L6 cell lysate, mouse liver tissue.

偶联

unconjugated

克隆号

SI15-04

RRID

AB_3069735

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:2,000

  • IHC-P

  • 1:2,000

  • IP

  • 1-2μg/sample

  • IF-Tissue

  • 1:1,000

靶点

功能

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Activation of MMP-2 requires proteolytic processing. A complex of membrane type 1 MMP (MT1-MMP/MMP14) and tissue inhibitor of metalloproteinase 2 recruits pro-MMP 2 from the extracellular milieu to the cell surface. Activation then requires an active molecule of MT1-MMP and auto catalytic cleavage. Clustering of integrin chains promotes activation of MMP-2. Another factor that will support the activation of MMP-2 is cell-cell clustering. A wild-type activated leukocyte cell adhesion molecule (ALCAM) is also required to activate MMP-2.

背景文献

1. Kim BJ et al. Expression of matrix metalloproteinase-2 and -9 in human ligamentum flavum cells treated with tumor necrosis factor-a and interleukin-1 . J Neurosurg Spine 24:428-35 (2016).

2. Dai X et al. SMAD3 deficiency promotes vessel wall remodeling, collagen fiber reorganization and leukocyte infiltration in an inflammatory abdominal aortic aneurysm mouse model. Sci Rep 5:10180 (2015).

序列相似性

Belongs to the peptidase M10A family.

组织特异性

Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.

翻译后修饰

Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.; The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.

亚细胞定位

Secreted, extracellular space, extracellular matrixm Membrane, Nucleus; Cytoplasm, Mitochondrion.

UNIPROT

SwissProt: P08253 Human

SwissProt: P33434 Mouse

SwissProt: P33436 Rat

别名

72 kDa gelatinase antibody

72kD type IV collagenase antibody

CLG 4 antibody

CLG 4A antibody

CLG4 antibody

CLG4A antibody

Collagenase Type 4 alpha antibody

Collagenase type IV A antibody

Gelatinase A antibody

Gelatinase alpha antibody

展开

图片

  • Western blot analysis of MMP-2 on different lysates with Rabbit anti-MMP-2 antibody (<a href="/products/ET1606-4" style="font-weight: bold;text-decoration: underline;">ET1606-4</a>) at 1/2,000 dilution.<br /><br />Lane 1: U-87 MG cell lysate<br />Lane 2: L6 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 74 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 3 minutes 30 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1606-4" style="font-weight: bold;text-decoration: underline;">ET1606-4</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MMP-2 on different lysates with Rabbit anti-MMP-2 antibody (ET1606-4) at 1/2,000 dilution.

    Lane 1: U-87 MG cell lysate
    Lane 2: L6 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 74 kDa
    Observed band size: 70 kDa

    Exposure time: 3 minutes 30 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-MMP-2 antibody (<a href="/products/ET1606-4" style="font-weight: bold;text-decoration: underline;">ET1606-4</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1606-4" style="font-weight: bold;text-decoration: underline;">ET1606-4</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-MMP-2 antibody (ET1606-4) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-4) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MMP-2 was immunoprecipitated from 0.2 mg U-87 MG cell lysate with <a href="/products/ET1606-4" style="font-weight: bold;text-decoration: underline;">ET1606-4</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/ET1606-4" style="font-weight: bold;text-decoration: underline;">ET1606-4</a> at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: U-87 MG cell lysate (input)<br />Lane 2: <a href="/products/ET1606-4" style="font-weight: bold;text-decoration: underline;">ET1606-4</a> IP in U-87 MG cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/ET1606-4" style="font-weight: bold;text-decoration: underline;">ET1606-4</a> in U-87 MG cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 59 seconds; ECL: K1801

    MMP-2 was immunoprecipitated from 0.2 mg U-87 MG cell lysate with ET1606-4 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1606-4 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: U-87 MG cell lysate (input)
    Lane 2: ET1606-4 IP in U-87 MG cell lysate
    Lane 3: Rabbit IgG instead of ET1606-4 in U-87 MG cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 59 seconds; ECL: K1801

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: liver<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Mouse

    Site: liver

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IHC-P
  3. IF
  4. IF-cell

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