MLH1 Mouse Monoclonal Antibody [A10A3]
Catalog# HA601184
MLH1 Mouse Monoclonal Antibody [A10A3]
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WB
-
IHC
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Human
概述
产品名称
MLH1 Mouse Monoclonal Antibody [A10A3]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within human MLH1 aa 301-600 / 756.
种属反应性
Human
验证应用
WB, IHC
分子量
Predicted band size: 85 kDa
阳性对照
A431 cell lysate, HeLa cell lysate, Daudi cell lysate, K-562 cell lysate, HL-60 cell lysate, SW480 cell lysate, human appendix tissue, human tonsil tissue.
偶联
unconjugated
克隆号
A10A3
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
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IHC
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1:1,000
靶点
功能
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
背景文献
1. Huang Y.et al. 2022 Expressing MLH1 in HCT116 cells increases cellular resistance to radiation by activating the PRKAC. Exp Biol Med (Maywood). 247(5):426-432.
2. Manzoor S. et al. 2022. MLH1 mediates cytoprotective nucleophagy to resist 5-Fluorouracil-induced cell death in colorectal carcinoma. Neoplasia. 24(2):76-85.
亚细胞定位
Nucleus, Chromosome.
UNIPROT
别名
COCA 2 antibody
COCA2 antibody
DNA mismatch repair protein Mlh1 antibody
FCC 2 antibody
FCC2 antibody
hMLH 1 antibody
hMLH1 antibody
HNPCC 2 antibody
HNPCC antibody
HNPCC2 antibody
展开图片
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Western blot analysis of MLH1 on different lysates with Mouse anti-MLH1 antibody (HA601184) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Daudi cell lysate
Lane 4: K-562 cell lysate
Lane 5: HL-60 cell lysate
Lane 6: SW480 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84.6 kDa
Observed band size: 85 kDa
Exposure time: 1 minutes 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601184) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-MLH1 antibody (HA601184) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601184) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-MLH1 antibody (HA601184) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601184) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"